Abstract
Parathyroid hormone (PTH)/parathyroid hormone-related peptide (PTHrP) receptor expressed in rat cultured cortical type I astrocytes was identified and characterized. Northern blot analysis demonstrated that the PTH/PTHrP receptor mRNA is expressed in astrocytes. A human PTH fragment, PTH [1-34], stimulated adenylate cyclase with the EC50 value of 3 nM, but not phospholipase C. PTH [1-34] changed the morphology of protoplasmic type astrocytes into process-bearing ones. Thus, type I astrocytes possess PTH/PTHrP receptor that is coupled to an adenylate cyclase-activating system but not to phospholipase C.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenylyl Cyclases / metabolism
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Animals
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Astrocytes / drug effects
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Astrocytes / metabolism*
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Cells, Cultured
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Cyclic AMP / metabolism
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Humans
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Hydrolysis
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Parathyroid Hormone / pharmacology
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Parathyroid Hormone-Related Protein*
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Peptide Fragments / pharmacology
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Phosphatidylinositols / metabolism
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Proteins / pharmacology
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Receptor, Parathyroid Hormone, Type 1
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Receptors, Parathyroid Hormone / drug effects
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Receptors, Parathyroid Hormone / genetics
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Receptors, Parathyroid Hormone / metabolism*
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Teriparatide
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Type C Phospholipases / metabolism
Substances
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Parathyroid Hormone
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Parathyroid Hormone-Related Protein
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Peptide Fragments
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Phosphatidylinositols
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Proteins
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RNA, Messenger
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Receptor, Parathyroid Hormone, Type 1
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Receptors, Parathyroid Hormone
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Teriparatide
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parathyroid hormone-related protein (1-34)
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Cyclic AMP
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Type C Phospholipases
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Adenylyl Cyclases