Abstract
Prostaglandin E2 (PGE2) is a potent mediator in many human tissues, that is recognized by three distinct subtypes of receptors, designated EP1, EP2 and EP3. A cDNA from a human lung library encodes a 53 kDa protein of 88% homology with the mouse EP2 receptor. Human EP2 receptors in COS-7 cell transfectants bound [3H]-PGE2 with a mean Kd of 2.2 nM and native specificity, and transduced increases in the intra-cellular concentration of cyclic AMP, but not of Ca++. That most EP2 receptor mRNA is in lung, kidney, intestinal, glandular and immune tissues, is consistent with functional responses.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Calcium / metabolism
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Cells, Cultured
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Cloning, Molecular
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Cyclic AMP / metabolism
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DNA, Complementary / genetics
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Dinoprostone / metabolism*
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Dose-Response Relationship, Drug
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Gene Library
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Humans
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Molecular Sequence Data
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Phosphatidylinositols / metabolism
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Receptors, Prostaglandin E / biosynthesis
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Receptors, Prostaglandin E / classification
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Receptors, Prostaglandin E / genetics*
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Recombinant Proteins / biosynthesis
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Sequence Homology, Amino Acid
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Tissue Distribution
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Transfection
Substances
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DNA, Complementary
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Phosphatidylinositols
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Receptors, Prostaglandin E
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Recombinant Proteins
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Cyclic AMP
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Dinoprostone
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Calcium
Associated data
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GENBANK/D17642
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GENBANK/D17795
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GENBANK/D17796
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GENBANK/D17797
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GENBANK/D17798
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GENBANK/D17799
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GENBANK/D17800
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GENBANK/D17801
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GENBANK/D17802
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GENBANK/L25124