Abstract
Lectin affinity chromatography was used to purify in a single step the envelope glycoproteins of HIV-1, HIV-2, and SIV. Envelope glycoproteins carry the major determinants essential for protection by the humoral immune response. The purification of these proteins has previously been a laborious procedure. The glycoproteins were purified by a one-step procedure to a high level of purity by using Galanthus nivalis agglutinin (GNA). The purified glycoprotein had CD4-binding and antigenic reactivities. Strong immune responses to envelope proteins and peptides were seen in mice and primates after immunization with these preparations.
MeSH terms
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Animals
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Antibodies, Viral / biosynthesis
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Antigens, Viral / isolation & purification
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CD4 Antigens
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Galanthus
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Gene Products, env / immunology
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Gene Products, env / isolation & purification*
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HIV Antibodies / biosynthesis
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HIV Antigens / isolation & purification
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HIV-1 / immunology*
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HIV-2 / immunology*
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Humans
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Lectins
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Macaca fascicularis
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Mannose-Binding Lectins*
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Mice
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Mice, Inbred BALB C
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Neutralization Tests
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Plant Lectins
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Simian Immunodeficiency Virus / immunology*
Substances
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Antibodies, Viral
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Antigens, Viral
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CD4 Antigens
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Gene Products, env
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HIV Antibodies
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HIV Antigens
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Lectins
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Mannose-Binding Lectins
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Plant Lectins
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snowdrop lectin