Abstract
Ca-binding protein 2 (CaBP2) has been described previously as an intracisternal calcium-binding microsomal glycoprotein. We report now the primary sequence of this protein as deduced from the corresponding cDNA. The protein possesses a C-terminal -KEEL retention sequence and three repeats of the thioredoxin-like motive -EFYAPNCGHCK-, and represents the rat homolog of ERp72. In contrast to earlier reports on ERp72, CaBP2 possesses significant proteindisulfide isomerase activity. Furthermore, in contrast to ERp72, CaBP2 is a glycoporotein containing O-linked glycans. The amount of CaBP2 in H-35 Reuber hepatoma cells increases in parallel with that of immunoglobin heavy-chain-binding protein under conditions which lead to impaired glycosylation, while the amount of calreticulin, another KDEL-containing glycoprotein, remains almost unchanged.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Calcium-Binding Proteins / genetics*
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Calcium-Binding Proteins / isolation & purification
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Calcium-Binding Proteins / metabolism*
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Cloning, Molecular
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Gene Library
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Insulin / metabolism
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Isomerases / genetics*
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Isomerases / isolation & purification
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Isomerases / metabolism*
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Kinetics
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Liver / physiology
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Liver Neoplasms, Experimental / metabolism*
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Male
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Membrane Glycoproteins / genetics*
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Methionine / metabolism
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Microsomes, Liver / metabolism*
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Protein Disulfide-Isomerases
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Rats
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Rats, Wistar
Substances
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Calcium-Binding Proteins
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Insulin
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Membrane Glycoproteins
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Oligodeoxyribonucleotides
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endoplasmic reticulum glycoprotein p72
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Methionine
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Isomerases
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Protein Disulfide-Isomerases
Associated data
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GENBANK/D14867
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GENBANK/D14868
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GENBANK/L09679
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GENBANK/L09680
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GENBANK/L09681
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GENBANK/L09682
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GENBANK/L09683
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GENBANK/M86870
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GENBANK/X57950
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GENBANK/X71000