Abstract
To test the suggested structural relationship between the electrogenic H+ transporting system and the NADPH oxidase of phagocytes, the existence of the enzyme and the transport process was investigated in human tonsillar T lymphocytes. It is shown that tonsillar T cells possess an arachidonic acid activatable, Cd(2+)- and Zn(2+)-sensitive electrogenic H+ efflux pathway with similar properties as reported earlier in various phagocytic cells. The presence of cytochrome b558, the membrane component of the oxidase, could not be detected in tonsillar T lymphocytes either by immunoblot or by flow cytometric analysis. It is suggested that the electrogenic H+ transporting pathway is structurally independent of the NADPH oxidase complex.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Arachidonic Acid / pharmacology*
-
Cadmium / pharmacology
-
Cytochrome b Group / analysis
-
Cytochrome b Group / deficiency
-
Cytochrome b Group / metabolism*
-
Dicyclohexylcarbodiimide / pharmacology
-
Flow Cytometry
-
Granulocytes / immunology
-
Granulocytes / metabolism
-
Humans
-
Kinetics
-
Masoprocol / pharmacology
-
NADH, NADPH Oxidoreductases / metabolism
-
NADPH Oxidases
-
Palatine Tonsil
-
Proton-Translocating ATPases / drug effects
-
Proton-Translocating ATPases / metabolism*
-
T-Lymphocytes / drug effects
-
T-Lymphocytes / immunology
-
T-Lymphocytes / metabolism*
-
Zinc / pharmacology
Substances
-
Cytochrome b Group
-
Cadmium
-
Arachidonic Acid
-
Dicyclohexylcarbodiimide
-
Masoprocol
-
cytochrome b558
-
NADH, NADPH Oxidoreductases
-
NADPH Oxidases
-
Proton-Translocating ATPases
-
Zinc