Characterization of a new peptide from Tityus serrulatus scorpion venom which is a ligand of the apamin-binding site

FEBS Lett. 1996 Jul 15;390(1):81-4. doi: 10.1016/0014-5793(96)00616-3.

Abstract

A new ligand (Ts kappa) of the apamin binding site on rat brain synaptosomes (K0.5 = 300 pM) was purified and characterized from the venom of Tityus serrulatus. It is a polypeptide toxin of 35 amino acid residues, with three disulfide bridges. Its cDNA was amplified from a venom gland cDNA library and the nucleotide sequence determined. A model of Ts kappa was constructed by amino acid replacement using charybdotoxin structure as determined by 1H nuclear magnetic resonance as starting model.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Apamin / metabolism
  • Base Sequence
  • Brain / metabolism*
  • DNA Primers
  • DNA, Complementary
  • Ligands
  • Models, Molecular
  • Molecular Sequence Data
  • Neurotoxins / chemistry
  • Neurotoxins / isolation & purification
  • Neurotoxins / metabolism
  • Peptides / chemistry
  • Peptides / isolation & purification
  • Peptides / metabolism
  • Polymerase Chain Reaction
  • Potassium Channels / drug effects
  • Potassium Channels / metabolism*
  • Potassium Channels / physiology*
  • Protein Conformation
  • Rats
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Scorpion Venoms / chemistry*
  • Scorpion Venoms / isolation & purification
  • Scorpion Venoms / metabolism*
  • Scorpions
  • Sequence Homology, Amino Acid
  • Synaptosomes / metabolism*

Substances

  • DNA Primers
  • DNA, Complementary
  • Ligands
  • Neurotoxins
  • Peptides
  • Potassium Channels
  • Recombinant Proteins
  • Scorpion Venoms
  • apamin receptor
  • Apamin
  • tityustoxin