Abstract
A new ligand (Ts kappa) of the apamin binding site on rat brain synaptosomes (K0.5 = 300 pM) was purified and characterized from the venom of Tityus serrulatus. It is a polypeptide toxin of 35 amino acid residues, with three disulfide bridges. Its cDNA was amplified from a venom gland cDNA library and the nucleotide sequence determined. A model of Ts kappa was constructed by amino acid replacement using charybdotoxin structure as determined by 1H nuclear magnetic resonance as starting model.
MeSH terms
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Amino Acid Sequence
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Animals
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Apamin / metabolism
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Base Sequence
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Brain / metabolism*
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DNA Primers
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DNA, Complementary
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Ligands
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Models, Molecular
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Molecular Sequence Data
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Neurotoxins / chemistry
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Neurotoxins / isolation & purification
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Neurotoxins / metabolism
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Peptides / chemistry
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Peptides / isolation & purification
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Peptides / metabolism
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Polymerase Chain Reaction
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Potassium Channels / drug effects
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Potassium Channels / metabolism*
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Potassium Channels / physiology*
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Protein Conformation
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Rats
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Recombinant Proteins / chemistry
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Scorpion Venoms / chemistry*
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Scorpion Venoms / isolation & purification
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Scorpion Venoms / metabolism*
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Scorpions
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Sequence Homology, Amino Acid
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Synaptosomes / metabolism*
Substances
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DNA Primers
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DNA, Complementary
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Ligands
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Neurotoxins
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Peptides
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Potassium Channels
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Recombinant Proteins
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Scorpion Venoms
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apamin receptor
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Apamin
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tityustoxin