Abstract
Hepatic apolipoprotein (apo) B RNA editing was examined in the genetically obese hyperinsulinemic and hypertriglyceridemic Zucker rat. In obese Zucker rats, apo B RNA editing was increased 42% relative to that in lean controls. Correspondingly, the proportion of serum triglyceride-rich lipoprotein containing apo B48 increased 4.7-fold in the obese Zucker rat. Quantification of hepatic total apo B mRNA showed no difference between obese Zucker and lean control rats. In contrast, the hepatic mRNA encoding APOBEC-1, the catalytic subunit of the RNA editing activity, demonstrated an increased abundance of 1.8-fold in obese Zucker rats versus lean controls.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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APOBEC-1 Deaminase
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Animals
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Apolipoproteins B / genetics*
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Cytidine Deaminase / genetics
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Cytidine Deaminase / metabolism
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Disease Models, Animal
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Hyperinsulinism / blood
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Hyperinsulinism / genetics
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Hyperinsulinism / metabolism
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Hypertriglyceridemia / blood
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Hypertriglyceridemia / genetics
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Hypertriglyceridemia / metabolism
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Insulin Resistance / genetics
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Liver / metabolism*
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Male
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Obesity / genetics
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Obesity / metabolism*
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RNA Editing*
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RNA, Messenger / genetics
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Rats
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Rats, Zucker
Substances
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Apolipoproteins B
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RNA, Messenger
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AICDA (activation-induced cytidine deaminase)
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APOBEC-1 Deaminase
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Apobec1 protein, rat
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Cytidine Deaminase