Cloning and expression of solanidine UDP-glucose glucosyltransferase from potato

Plant J. 1997 Feb;11(2):227-36. doi: 10.1046/j.1365-313x.1997.11020227.x.

Abstract

A cDNA encoding solanidine glucosyltransferase (SGT) was isolated from potato. The cDNA was selected from a yeast expression library using a positive selection based on the higher toxicity of steroidal alkaloid aglycons relative to their associated glycosylated forms. The cDNA contained an open reading frame encoding a 56 kDa polypeptide with regions of similarity to previously characterized UDP-glucosyltransferases. The enzyme activity and reaction products of recombinant SGT in yeast were consistent with those observed for the endogenous enzyme from potato. SGT mRNA and protein accumulated in tubers in response to wounding. The time course for SGT mRNA accumulation paralleled that of 3-hydroxy-3-methylglutaryl-coenzymeA isoform 1 (hmg1) mRNA. Steady-state SGT mRNA levels also increased transiently upon wounding of leaves.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Carboxylic Ester Hydrolases*
  • Cloning, Molecular / methods
  • Escherichia coli / genetics
  • Gene Expression Regulation, Plant / physiology*
  • Glucosyltransferases / genetics*
  • Hydroxymethylglutaryl CoA Reductases / genetics
  • Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent
  • Molecular Sequence Data
  • Plant Proteins / genetics
  • RNA, Messenger / analysis
  • RNA, Plant / analysis
  • Recombinant Proteins
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics
  • Sequence Analysis, DNA
  • Solanaceous Alkaloids / metabolism
  • Solanaceous Alkaloids / pharmacology
  • Solanum tuberosum / enzymology
  • Solanum tuberosum / genetics*
  • Substrate Specificity
  • Transcriptional Activation

Substances

  • Plant Proteins
  • RNA, Messenger
  • RNA, Plant
  • Recombinant Proteins
  • Solanaceous Alkaloids
  • patatin protein, Solanum tuberosum
  • Hydroxymethylglutaryl CoA Reductases
  • Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent
  • Glucosyltransferases
  • solanidine glucosyltransferase
  • Carboxylic Ester Hydrolases

Associated data

  • GENBANK/A54739
  • GENBANK/B53652
  • GENBANK/P16662
  • GENBANK/P19488
  • GENBANK/P36538
  • GENBANK/S33169
  • GENBANK/S41951
  • GENBANK/U82367