Activation of mitogen-activated protein kinase by the nociceptin receptor expressed in Chinese hamster ovary cells

FEBS Lett. 1997 Jul 28;412(2):290-4. doi: 10.1016/s0014-5793(97)00815-6.

Abstract

Activation of the nociceptin receptor stably expressed in Chinese hamster ovary cells induced a transient mitogen-activated protein kinase (MAPK) activation, via pertussis toxin-sensitive G-proteins. The nociceptin receptor-mediated MAPK activation was partially blocked by down-regulation or inhibition of protein kinase C, and suppressed by pretreatment with a phosphatidylcholine-specific phospholipase C inhibitor, D609. Furthermore, a tyrosine protein kinase inhibitor, genistein, and phosphatidylinositol 3-kinase inhibitors, wortmannin and LY294002, affected the nociceptin-induced MAPK activity. The nociceptin-induced MAPK activation may lead to activation of phospholipase A2 and induce changes in gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Androstadienes / pharmacology
  • Animals
  • CHO Cells
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism*
  • Chromones / pharmacology
  • Cricetinae
  • Cricetulus
  • Down-Regulation
  • Enzyme Activation
  • Enzyme Inhibitors / pharmacology
  • Indoles / pharmacology
  • Maleimides / pharmacology
  • Mitogen-Activated Protein Kinase 1
  • Mitogen-Activated Protein Kinase 3
  • Mitogen-Activated Protein Kinases*
  • Morpholines / pharmacology
  • Nociceptin Receptor
  • Phosphatidylinositol 3-Kinases
  • Phosphotransferases (Alcohol Group Acceptor) / antagonists & inhibitors
  • Protein Binding
  • Protein Kinase C / antagonists & inhibitors
  • Protein Kinase C / metabolism
  • Protein-Tyrosine Kinases / metabolism*
  • Receptors, Opioid / genetics
  • Receptors, Opioid / metabolism*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Tetradecanoylphorbol Acetate / pharmacology
  • Wortmannin

Substances

  • Androstadienes
  • Chromones
  • Enzyme Inhibitors
  • Indoles
  • Maleimides
  • Morpholines
  • Receptors, Opioid
  • Recombinant Proteins
  • 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
  • Phosphatidylinositol 3-Kinases
  • Phosphotransferases (Alcohol Group Acceptor)
  • Protein-Tyrosine Kinases
  • Protein Kinase C
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Mitogen-Activated Protein Kinase 1
  • Mitogen-Activated Protein Kinase 3
  • Mitogen-Activated Protein Kinases
  • bisindolylmaleimide I
  • Tetradecanoylphorbol Acetate
  • Wortmannin
  • Nociceptin Receptor