Isolation and cloning of a Drosophila homolog to the mammalian RACK1 gene, implicated in PKC-mediated signalling

Biochim Biophys Acta. 1997 Aug 21;1358(1):67-71. doi: 10.1016/s0167-4889(97)00079-7.

Abstract

The mammalian RACK1 protein binds activated protein kinase C, acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. Genes encoding RACK1-like proteins have been isolated from a wide range of eucaryotic organisms; we report the isolation of a Drosophila member of this family. This Drosophila RACK1-like protein shows 76% identity to the mammalian RACK1 proteins, but only about 60% identity to related proteins from plants and fungi. The Drosophila rack1 gene has a dynamic pattern of expression during early embryogenesis with the highest expression in the mesodermal and endodermal lineages.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Drosophila / embryology
  • Drosophila / genetics*
  • Genes, Insect*
  • Molecular Sequence Data
  • Peptides / genetics*
  • Protein Kinase C / genetics*
  • Protein Kinase C / metabolism
  • Receptors for Activated C Kinase
  • Sequence Homology, Amino Acid
  • Signal Transduction

Substances

  • Peptides
  • Receptors for Activated C Kinase
  • peptide I
  • Protein Kinase C

Associated data

  • GENBANK/U96491