Abstract
Antibodies designed to recognize a 74 amino acid sequence of the N- or C-terminal domain of the rat CB1 cannabinoid receptor detected a 58 kDa protein in immunoblots of brain and various cells known to express the CB1 cannabinoid receptor. A human B-lymphoblastoid cell line and macrophage-like cells from neonatal rat brain were also positive for CB1 receptor-like immunoreactivity. Immunocytochemical analysis performed with isolated Fab fragments showed surface staining in NG108-15 cells and brain macrophage like cells which also express MHC class II antigens. The data suggest a plausible role for CB1 receptors in the immune function of brain.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Animals, Newborn
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Antibody Specificity
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Brain / cytology
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Brain / immunology*
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Brain Chemistry / immunology
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CHO Cells
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Cannabinoids / genetics
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Cannabinoids / immunology
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Cricetinae
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Female
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GTP-Binding Proteins / immunology
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Gene Expression
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Macrophages / chemistry
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Macrophages / immunology
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Microglia / chemistry*
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Microglia / immunology
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Rabbits
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Rats
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Receptors, Cannabinoid
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Receptors, Drug / analysis
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Receptors, Drug / genetics*
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Receptors, Drug / immunology*
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Recombinant Fusion Proteins / immunology
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Schistosoma japonicum
Substances
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Cannabinoids
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Receptors, Cannabinoid
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Receptors, Drug
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Recombinant Fusion Proteins
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GTP-Binding Proteins