pHaMDR-DHFR bicistronic expression system for mutational analysis of P-glycoprotein

Methods Enzymol. 1998:292:474-80. doi: 10.1016/s0076-6879(98)92036-5.

Authors

S Zhang¹, Y Sugimoto, T Shoshani, I Pastan, M M Gottesman

Affiliation

¹ Division of Oncology, Hospital for Sick Children, Toronto, Ontario, Canada.

PMID: 9711575
DOI: 10.1016/s0076-6879(98)92036-5

No abstract available

MeSH terms

ATP Binding Cassette Transporter, Subfamily B, Member 1 / biosynthesis
ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism*
Cloning, Molecular / methods
DNA Mutational Analysis / methods
DNA Primers
Drug Resistance, Multiple
Genetic Vectors
Humans
KB Cells
Methotrexate / toxicity
Mutagenesis, Insertional
Mutagenesis, Site-Directed
Open Reading Frames
Polymerase Chain Reaction / methods
Recombinant Fusion Proteins / biosynthesis
Recombinant Fusion Proteins / metabolism
Tetrahydrofolate Dehydrogenase / biosynthesis
Tetrahydrofolate Dehydrogenase / metabolism
Transfection / methods

Substances

ATP Binding Cassette Transporter, Subfamily B, Member 1
DNA Primers
Recombinant Fusion Proteins
Tetrahydrofolate Dehydrogenase
Methotrexate