Drug-stimulatable ATPase activity in crude membranes of human MDR1-transfected mammalian cells

Methods Enzymol. 1998:292:504-14. doi: 10.1016/s0076-6879(98)92039-0.

¹ Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.

No abstract available

MeSH terms

3T3 Cells
ATP Binding Cassette Transporter, Subfamily B, Member 1 / biosynthesis
ATP Binding Cassette Transporter, Subfamily B, Member 1 / isolation & purification
ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism*
Adenosine Triphosphatases / biosynthesis
Adenosine Triphosphatases / isolation & purification
Adenosine Triphosphatases / metabolism*
Animals
Blotting, Western
Cell Membrane / metabolism*
Chromatography, High Pressure Liquid / methods
Drug Resistance, Multiple
Electrophoresis, Polyacrylamide Gel / methods
Humans
Indicators and Reagents
Kinetics
Mammals
Mice
Recombinant Proteins / biosynthesis
Recombinant Proteins / isolation & purification
Recombinant Proteins / metabolism
Transfection / methods
Verapamil / pharmacology
Vincristine / pharmacology