Abstract
Experimental autoimmune encephalomyelitis (EAE) is an investigator-initiated disorder that serves as an animal model for the common human demyelinating disease multiple sclerosis. Both diseases are typified by disseminated perivascular and submeningeal cuffs in the central nervous system (CNS). It was shown recently that chemokines are integral to the pathogenesis of EAE. In the present study we analyzed the gene expression of three chemokines, RANTES, MIP-1alpha and GRO-alpha, at the onset of acute EAE, and correlated that expression with the intensity of inflammatory changes in the CNS. We showed that all three chemokines are upregulated simultaneously with symptom onset of acute EAE, and that chemokine expression correlates with the intensity of inflammation in the CNS. This consistent relationship supports the hypothesis that chemokines are relevant to leukocyte accumulation in CNS parenchyma.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acute Disease
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Animals
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Brain Chemistry / immunology
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Chemokine CCL3
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Chemokine CCL4
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Chemokine CCL5 / genetics*
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Chemokine CCL5 / immunology
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Chemokine CXCL1
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Chemokines, CXC*
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Chemotactic Factors / genetics*
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Chemotactic Factors / immunology
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Encephalomyelitis, Autoimmune, Experimental / immunology*
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Female
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Gene Expression / immunology
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Growth Substances / genetics*
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Growth Substances / immunology
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Intercellular Signaling Peptides and Proteins*
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Liver / immunology
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Liver / metabolism
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Macrophage Inflammatory Proteins / genetics*
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Macrophage Inflammatory Proteins / immunology
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Mice
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Mice, Inbred Strains
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Monocytes / immunology
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Spinal Cord / chemistry
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Spinal Cord / immunology
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T-Lymphocytes / immunology
Substances
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CXCL1 protein, human
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Chemokine CCL3
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Chemokine CCL4
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Chemokine CCL5
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Chemokine CXCL1
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Chemokines, CXC
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Chemotactic Factors
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Cxcl1 protein, mouse
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Growth Substances
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Intercellular Signaling Peptides and Proteins
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Macrophage Inflammatory Proteins