Abstract
Sphingosine 1-phosphate (S1P) induces phospholipase C (PLC) activation and Ca2+ mobilization in many types of cells. We examined the possible involvement of Edg-3, one of the putative S1P receptors, in the phospholipase C (PLC)-Ca2+ system. S1P increased the cytoplasmic free Ca2+ concentration without detectable inositol phosphate production in vector-transfected CHO cells. In the Edg-3-transfected cells, however, the S1P-induced Ca2+ response was clearly enhanced, which was associated with a significant production of inositol phosphate. These S1P-induced responses in the Edg-3-transfected cells were inhibited by U73122, a potent PLC inhibitor. We conclude that Edg-3 may be one of the S1P receptors participating in the activation of the PLC-Ca2+ system.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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CHO Cells
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Calcium Signaling*
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Cricetinae
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Enzyme Activation
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I-kappa B Proteins*
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Inositol Phosphates / metabolism
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Lysophospholipids*
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NF-KappaB Inhibitor alpha
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Receptors, Cell Surface / metabolism*
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Receptors, Lysophospholipid
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Recombinant Proteins / metabolism
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Sphingosine / analogs & derivatives*
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Sphingosine / metabolism
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Transfection
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Type C Phospholipases / metabolism*
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Uridine Triphosphate / metabolism
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Virulence Factors, Bordetella
Substances
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DNA-Binding Proteins
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I-kappa B Proteins
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Inositol Phosphates
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Lysophospholipids
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Receptors, Cell Surface
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Receptors, Lysophospholipid
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Recombinant Proteins
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Virulence Factors, Bordetella
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NF-KappaB Inhibitor alpha
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sphingosine 1-phosphate
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Type C Phospholipases
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Sphingosine
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Uridine Triphosphate