Phosphorylation of adenylyl cyclase VI upon chronic delta-opioid receptor stimulation

Eur J Pharmacol. 1999 Jan 8;364(2-3):R1-3. doi: 10.1016/s0014-2999(98)00847-4.

Abstract

An immunoprecipitation method was used to measure [32P]phosphate incorporation into the adenylyl cyclase VI protein in Chinese Hamster Ovary (CHO) cells stably expressing the human delta-opioid receptor. Chronic SNC 80 ((+)-4-[(alpha R)-alpha-((2S,5R)-4-allyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]-N ,N-diethyl-benzamide) 1 microM, 24 h) treatment increased the incorporation of [32P] into a 200 kDa protein band 2.5-fold after gel electrophoresis. The increase in phosphorylation of adenylyl cyclase VI was antagonized by naltrindole (1 microM) and the immunoprecipitation was prevented by the saturation of the antibody with the blocking peptide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenylyl Cyclases / drug effects
  • Adenylyl Cyclases / metabolism*
  • Animals
  • Benzamides / pharmacology
  • CHO Cells / cytology
  • CHO Cells / drug effects
  • CHO Cells / metabolism
  • Cricetinae
  • Dose-Response Relationship, Drug
  • Naltrexone / analogs & derivatives
  • Naltrexone / pharmacology
  • Narcotic Antagonists / pharmacology
  • Phosphorylation / drug effects
  • Piperazines / pharmacology
  • Receptors, Opioid, delta / agonists
  • Receptors, Opioid, delta / antagonists & inhibitors
  • Receptors, Opioid, delta / metabolism*
  • Time Factors

Substances

  • Benzamides
  • Narcotic Antagonists
  • Piperazines
  • Receptors, Opioid, delta
  • 4-(alpha-(4-allyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl)-N,N-diethylbenzamide
  • Naltrexone
  • Adenylyl Cyclases
  • naltrindole