To determine the site and mechanism of action of gonadal steroids on pituitary nitric oxide
synthase type I (NOS I), present in both gonadotrophs and folliculo-stellate cells, the effects of …

We have recently provided evidence that the concentration and activity of the enzyme nitric
oxide synthase type I (NOS I) was stimulated in gonadotrophs by GnRH, suggesting a role for …

Increasing evidence exist that multiple G proteins mediate the effects of gonadotropin-releasing
hormone (GnRH) on the synthesis and release of pituitary gonadotropins. In the present …

Using single hormone-staining immunocytochemical methods, we have recently characterized
a novel cell line, RC-4B/C, established from an aged male rat pituitary adenoma. This cell …

Previous studies have established that the interaction of gonadotropin-releasing hormone (GnRH)
with its receptor (GnRHR) would require partial entry of the N- and C-terminal regions …

An epithelial cell line (RC-4B/C) was established from a pituitary adenoma obtained from a 3-yr-old
(ACI/fMai × F344/fMai)F1 male rat. Before Year 5 in vitro, RC-4B/C cells could not be …

The GnRH-R belongs to the superfamily of heptahelical GPCRs. A three-dimensional model
of GnRH binding to its receptor predicted that Trp3 was the most deeply buried residue, …

The high-affinity gonadoliberin (GnRH) receptor contained in a membrane preparation from
frozen bovine anterior pituitary glands has been solubilized in Triton X-100 and the binding …

To further explore the mechanism of steroid feedback in male, the effects of testosterone (T)
and gonadotropin-releasing hormone (GnRH) on the rates of α- and lutropin (LH)β-chain …

The plasma membranes of many tissues are considered as the site at which the hormone
initiates its action. It has thus been suggested that the anterior pituitary plasma membranes …