… denaturation step at 95C for 5 minutes, 30 cycles were performed (1 minute at 95C, 1 minute
at 60C, and 1 minute at 72C) followed by a final 10-minute extension step at 72C. The PCR …

Incubation of human A549/8 cells with human interleukin-1 beta (50 units/ml), interferon-gamma
(100 units/ml), and tumor necrosis factor-alpha (10 ng/ml) (cytomix) resulted in a marked …

… Reverse transcription reaction was incubated at 50C for 30 min. PCR amplification with … C
for 2 min. The samples were then denatured at 95C for 1 min, annealed, and extended at 72C …

Transcription factor NF-κB is essential for the induction of nitric oxide synthase (NOS) II (iNOS)
by bacterial lipopolysaccharide in murine macrophages (Xie, QW, Kashiwabara, Y., and …

… 30 C, ramped to 40 C with 5 C/min, ramped to 200 C with 10 C/min, then ramped to the final
temperature of 250 C with 5 C/… was 250 C and the quadrupole was set to 150 C. The filament …

… Human blood from volunteers was drawn and allowed to clot at 37C for 1 h in the
presence of increasing concentrations of the test compounds (R‐ibuprofen, S‐ibuprofen, R‐…

… Thermal cycler parameters were 2 min at 50C, 10 min at 95C, and then 45 cycles of [15 s
at 95C and 1 min at 60C]. Primers and TaqMan probes were synthesized by TIB MOLBIOL (…

Prostaglandins are important mediators in spinal nociceptive processing. They are produced
by cyclo-oxygenase isoforms, cyclo-oxygenase-1 and -2, which are both constitutively …

The localization and regulation of neuronal nitric oxide synthase (nNOS), inducible nitric oxide
synthase (iNOS) and soluble guanylyl cyclase (sGC) were assessed in the spinal cord of …

Prostaglandins (PGs) are known to be involved in inflammatory and nociceptive processing.
Since the discovery of at least two isozymes of cyclooxygenase (COX), inhibition of COX-2 …