Measurement of the millisecond activation switch of G protein–coupled receptors in living cells
JP Vilardaga, M Bünemann, C Krasel, M Castro… - Nature …, 2003 - nature.com
… To this end, we coexpressed functional PTHR carrying CFP at its C terminus (PTHR-CFP
C-term ) and β-arrestin2 fused at its C terminus to YFP (β-arrestin2-YFP). We monitored PTH-…
C-term ) and β-arrestin2 fused at its C terminus to YFP (β-arrestin2-YFP). We monitored PTH-…
μ-opioid receptors: correlation of agonist efficacy for signalling with ability to activate internalization
…, M Baptist, J Llorente, S Al-Sabah, C Krasel… - Molecular …, 2010 - ASPET
We have compared the ability of a number of μ-opioid receptor (MOPr) ligands to activate G
proteins with their abilities to induce MOPr phosphorylation, to promote association of …
proteins with their abilities to induce MOPr phosphorylation, to promote association of …
Low intrinsic efficacy for G protein activation can explain the improved side effect profiles of new opioid agonists
…, NL Grimsey, M Kassiou, B Kellam, C Krasel… - Science …, 2020 - science.org
… Similar to most other G protein–coupled receptors (GPCRs), MOR signaling is regulated
by the phosphorylation of intracellular C-terminal serine and threonine residues, which …
by the phosphorylation of intracellular C-terminal serine and threonine residues, which …
[HTML][HTML] β-Arrestin binding to the β2-adrenergic receptor requires both receptor phosphorylation and receptor activation
C Krasel, M Bünemann, K Lorenz… - Journal of Biological …, 2005 - ASBMB
… which phosphorylate serine and threonine residues in the C terminus or the intracellular
loops of the receptors. The phosphorylated receptors then bind β-arrestins, and this interaction …
loops of the receptors. The phosphorylated receptors then bind β-arrestins, and this interaction …
Involvement of Asn-293 in stereospecific agonist recognition and in activation of the beta 2-adrenergic receptor.
K Wieland, HM Zuurmond, C Krasel… - Proceedings of the …, 1996 - National Acad Sciences
… as described earlier (27) using an incubation time of 1 hr at 30C. Saturation studies were done
with radioligand concentrations from 2 pM to … The incubation was done for 30 min at 30C. …
with radioligand concentrations from 2 pM to … The incubation was done for 30 min at 30C. …
[HTML][HTML] β-arrestin1 and 2 exhibit distinct phosphorylation-dependent conformations when coupling to the same GPCR in living cells
…, A Chevigné, C Krasel, X Deupi, C Hoffmann - Nature …, 2022 - nature.com
… In the ‘hanging’ complex configuration β-arrestins mostly interact with negatively charged or
phosphorylated residues of the receptor C-terminus 16 , thus we can interpret the measured …
phosphorylated residues of the receptor C-terminus 16 , thus we can interpret the measured …
GABA and glycine in synaptic vesicles: storage and transport characteristics
PM Burger, J Hell, E Mehl, C Krasel, F Lottspeich… - Neuron, 1991 - cell.com
… by isolating vesicles at 21C (see [A] and text). The figure shows mean values (+ … in vitro and
then maintained at 0C in the presence … control, vesicles were isolated at V’CIOT or at 21C. …
then maintained at 0C in the presence … control, vesicles were isolated at V’CIOT or at 21C. …
Multisite phosphorylation is required for sustained interaction with GRKs and arrestins during rapid μ-opioid receptor desensitization
…, M Göldner, JG Ruland, M Bünemann, C Krasel… - Science …, 2018 - science.org
… and 370 TREHPSTANT 379 within its C-terminal region—that … ; multisite phosphorylation
in the C-terminal region of MOR … We have previously shown that C-terminal phosphorylation …
in the C-terminal region of MOR … We have previously shown that C-terminal phosphorylation …
Protein kinase cross-talk: membrane targeting of the beta-adrenergic receptor kinase by protein kinase C.
R Winstel, S Freund, C Krasel… - Proceedings of the …, 1996 - National Acad Sciences
… is subject to phosphorylation and regulation by protein kinase C (PKC). In cell lines stably
… The site of phosphorylation was mapped to the C terminus of betaARK-1. We conclude that …
… The site of phosphorylation was mapped to the C terminus of betaARK-1. We conclude that …
Endomorphin-2: a biased agonist at the μ-opioid receptor
…, EM Rosethorne, SJ Charlton, C Krasel… - Molecular …, 2012 - ASPET
… For time course experiments, cells were washed and then challenged for 0 to 30 min at 37C
… antibody for 1 h at 4C before incubation with the agonists for 30 min at 37C. Cells were then …
… antibody for 1 h at 4C before incubation with the agonists for 30 min at 37C. Cells were then …