User profiles for David J. Segal

David J. Segal

University of California, Davis
Verified email at ucdavis.edu
Cited by 12183

Stimulation of homologous recombination through targeted cleavage by chimeric nucleases

…, D Carroll, DJ Segal, JK Trautman, J Smith… - … and cellular biology, 2001 - Taylor & Francis
Chimeric nucleases that are hybrids between a nonspecific DNA cleavage domain and a
zinc finger DNA recognition domain were tested for their ability to find and cleave their target …

Toward controlling gene expression at will: Specific regulation of the erbB-2/HER-2 promoter by using polydactyl zinc finger proteins constructed from modular …

RR Beerli, DJ Segal, B Dreier… - Proceedings of the …, 1998 - National Acad Sciences
To create a universal system for the control of gene expression, we have studied methods
for the construction of novel polydactyl zinc finger proteins that recognize extended DNA …

Structure-based redesign of the dimerization interface reduces the toxicity of zinc-finger nucleases

…, V Brondani, J Büchel, L Serrano, DJ Segal… - Nature …, 2007 - nature.com
Artificial endonucleases consisting of a FokI cleavage domain tethered to engineered zinc-finger
DNA-binding proteins have proven useful for stimulating homologous recombination in …

Toward controlling gene expression at will: selection and design of zinc finger domains recognizing each of the 5′-GNN-3′ DNA target sequences

DJ Segal, B Dreier, RR Beerli… - Proceedings of the …, 1999 - National Acad Sciences
We have taken a comprehensive approach to the generation of novel DNA binding zinc finger
domains of defined specificity. Herein we describe the generation and characterization of …

Design of polydactyl zinc-finger proteins for unique addressing within complex genomes

Q Liu, DJ Segal, JB Ghiara… - Proceedings of the …, 1997 - National Acad Sciences
Zinc-finger proteins of the Cys 2 -His 2 type represent a class of malleable DNA-binding
proteins that may be selected to bind diverse sequences. Typically, zinc-finger proteins …

Keep your fingers off my DNA: protein–protein interactions mediated by C2H2 zinc finger domains

KJ Brayer, DJ Segal - Cell biochemistry and biophysics, 2008 - Springer
Cys2-His2 (C2H2) zinc finger domains (ZFs) were originally identified as DNA-binding
domains, and uncharacterized domains are typically assumed to function in DNA binding. …

[HTML][HTML] Development of zinc finger domains for recognition of the 5′-ANN-3′ family of DNA sequences and their use in the construction of artificial transcription …

B Dreier, RR Beerli, DJ Segal, JD Flippin… - Journal of Biological …, 2001 - ASBMB
In previous studies we have developed Cys 2 -His 2 zinc finger domains that specifically
recognized each of the 16 5′-GNN-3′ DNA target sequences and could be used to assemble …

SRA-and SET-domain-containing proteins link RNA polymerase V occupancy to DNA methylation

…, X Zhong, G Marson, M Pellegrini, DJ Segal… - Nature, 2014 - nature.com
RNA-directed DNA methylation in Arabidopsis thaliana depends on the upstream synthesis
of 24-nucleotide small interfering RNAs (siRNAs) by RNA POLYMERASE IV (Pol IV) 1 , 2 …

[HTML][HTML] The NIH somatic cell genome editing program

…, V Sabbisetti, WM Saltzman, PJ Santangelo, DJ Segal… - Nature, 2021 - nature.com
The move from reading to writing the human genome offers new opportunities to improve
human health. The United States National Institutes of Health (NIH) Somatic Cell Genome …

A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture

…, MS Bhakta, JF Meckler, DJ Segal - Nucleic acids …, 2015 - academic.oup.com
Clustered regularly interspaced short palindromic repeat (CRISPR) RNA-guided nucleases
have gathered considerable excitement as a tool for genome engineering. However, …