Functions of heteromeric association between adenosine and P2Y receptors

H Nakata, K Yoshioka, T Kamiya, H Tsuga… - Journal of molecular …, 2005 - Springer
It is now well accepted that G protein-coupled receptors (GPCRs) can be directly associated,
as either homo-or hetero-oligomers, to alter their functions. G protein-coupled purinergic …

Regulation of pharmacology by hetero-oligomerization between A1 adenosine receptor and P2Y2 receptor

T Suzuki, K Namba, H Tsuga, H Nakata - Biochemical and biophysical …, 2006 - Elsevier
… binding sites (B max ) for both [ 3 H]CCPA and [ 3 H]DPCPX in the membranes from HA-A 1
… /P2Y 2 R-coexpressing cells, we examined [ 3 H]CCPA saturation binding in the presence or …

Sequestration of muscarinic acetylcholine receptor m2 subtypes. Facilitation by G protein-coupled receptor kinase (GRK2) and attenuation by a dominant-negative …

H Tsuga, K Kameyama, T Haga, H Kurose… - Journal of Biological …, 1994 - ASBMB
… ) for 1 h at room temperature and then with 1/2,000 diluted antiserum for 2 h at room temperature.
… , Inc.) in the blocking buffer for 1 h at room temperature. After removal of the secondary …

[HTML][HTML] Internalization and down-regulation of human muscarinic acetylcholine receptor m2 subtypes: role of third intracellular m2 loop and G protein-coupled …

H Tsuga, K Kameyama, T Haga, T Honma… - Journal of Biological …, 1998 - ASBMB
… [ 3 H]N-methylscopolamine binding sites from the cell surface, and transfer of [ 3 H]quinuclidinyl …
demonstrated by determining the loss of [ 3 H]quinuclidinyl benzilate binding sites in the …

Sequestration of the short and long isoforms of dopamine D2 receptors expressed in Chinese hamster ovary cells.

M Itokawa, M Toru, K Ito, H Tsuga, K Kameyama… - Molecular …, 1996 - ASPET
The short (D2S) and long (D2L) isoforms of dopamine D2 receptors were stably expressed
in Chinese hamster ovary cells, and dopamine-induced sequestration was examined by …

Sequestration of Human Muscarinic Acetylcholine Receptor hm1—hm5 Subtypes: Effect of G Protein-Coupled Receptor Kinases GRK2, GRK4, GRK5 and GRK6

H Tsuga, E Okuno, K Kameyama, T Haga - Journal of Pharmacology and …, 1998 - ASPET
… The [ 3 H]NMS binding activity of intact cells was measured as described previously (Tsuga
for three times and incubated with 1.2 to 1.6 nM [ 3 H]NMS in HEPES-buffered saline (25 mM …

Ca2+-Dependent Inhibition of G Protein-Coupled Receptor Kinase 2 by Calmodulin

K Haga, H Tsuga, T Haga - Biochemistry, 1997 - ACS Publications
H]-N-metylscopolamine ([ 3 H]NMS, a hydrophilic ligand) binding sites from the cell surface,
as described previously (Tsuga … or without 0.4 mM A23187 for 1 h. After the incubation, cells …

[HTML][HTML] Role of receptor kinase in short-term desensitization of cardiac muscarinic K+ channels expressed in Chinese hamster ovary cells

Z Shui, IA Khan, H Tsuga, T Haga… - The Journal of …, 1998 - ncbi.nlm.nih.gov
… Expression levels of stable transfected hm2 receptor were estimated using [ 3 H] QNB (…
antibodies (Tsuga et al. 1994). In the cell line stably transfected with just hm2, the [ 3 H] QNB …

G protein-coupled receptor kinase: phosphorylation of muscarinic receptors and facilitation of receptor sequestration

T Haga, K Haga, K Kameyama, H Tsuga - Advances in Pharmacology, 1996 - Elsevier
… of carbamylcholine but hardly phosphorylated in its absence (Tsuga et al., 1994). The agonist-…
mylcholine was assessed as the loss of [)H]NMS binding sites from cell surface. [From H. …

Ca2+/Calmodulin‐Mediated Regulation of Agonist‐Induced Sequestration of Gq Protein‐Coupled Histamine H1 Receptors in Human U373 MG Astrocytoma Cells

S Hishinuma, A Naiki, H Tsuga… - Journal of …, 1998 - Wiley Online Library
… ‐induced sequestration of H 1 receptors from the cell surface membrane was detected as
the loss of [ 3 H]mepyramine binding sites on intact cells accessible to the hydrophilic H 1 ‐…