Regeneration of nicotinamide cofactors for use in organic synthesis

HK Chenault, GM Whitesides - Applied biochemistry and biotechnology, 1987 - Springer
The high cost of nicotinamide cofactors requires that they be regeneratedin situ when used
in preparative enzymatic synthesis. Numerous strategies have been tested forin situ …

Kinetic resolution of unnatural and rarely occurring amino acids: enantioselective hydrolysis of N-acyl amino acids catalyzed by acylase I

HK Chenault, J Dahmer… - Journal of the American …, 1989 - ACS Publications
Acylase I (aminoacylase; A-acylamino-acid amidohydrolase, EC 3.5. 1.14, from porcine
kidney and the fungus Aspergillus) is a broadly applicable enzymatic catalyst for the kinetic …

[PDF][PDF] Cofactor regeneration for enzyme-catalysed synthesis

HK Chenault, ES Simon… - … and Genetic Engineering …, 1988 - Taylor & Francis
Cofactors are low-molecular-weight non-protein species that are essential participants in
enzyme-catalysed reactions. Certain cofactors (eg pyridoxal phosphate and thiamine …

Membrane-enclosed enzymic catalysis (MEEC): a useful, practical new method for the manipulation of enzymes in organic synthesis

MD Bednarski, HK Chenault, ES Simon… - Journal of the …, 1987 - ACS Publications
The above electron transfers are very fast in comparison with the more standard Co (II)/Co (III)
couples. 12 In both cases described herein the transfered electron occupies a-…

Characterization of star adhesive sealants based on PEG/dextran hydrogels

…, C Crespo, AB Ramos, HK Chenault… - Macromolecular …, 2009 - Wiley Online Library
Swellable PEG amine/dextran aldehyde composite materials are emerging as a controlled,
biocompatible tissue adhesive. We explain how preservation of natural tissue amines …

Polysaccharide-based tissue adhesives for sealing corneal incisions

SK Bhatia, SD Arthur, HK Chenault, GD Figuly… - Current eye …, 2007 - Taylor & Francis
Purpose: To investigate the ability of a novel polysaccharide-based tissue adhesive to seal
corneal incisions, and to determine the effect of the tissue adhesive on corneal endothelial …

Interactions of polysaccharide-based tissue adhesives with clinically relevant fibroblast and macrophage cell lines

SK Bhatia, SD Arthur, HK Chenault, GK Kodokian - Biotechnology letters, 2007 - Springer
The effects of polysaccharide-based tissue adhesives on cell survival and inflammatory cell
activation were determined using in vitro mouse cell cultures. Cytotoxicity of tissue …

Lactate dehydrogenase-catalyzed regeneration of NAD from NADH for use in enzyme-catalyzed synthesis

HK Chenault, GM Whitesides - Bioorganic Chemistry, 1989 - Elsevier
Lactate dehydrogenase (LDH, rabbit muscle, EC 1.1.1.27), coupled with pyruvic and glyoxylic
acids as oxidants, regenerates NAD in situ for enzyme-catalyzed oxidations. The use of …

The chemistry of isopropenyl glycopyranosides. Transglycosylations and other reactions

HK Chenault, A Castro, LF Chafin… - The Journal of Organic …, 1996 - ACS Publications
Various anomerically pure isopropenyl α- and β-glycopyranosides have been synthesized
and shown to undergo synthetically useful transglycosylation reactions with a variety of …

Glycosyl transfer by isopropenyl glycosides: trisaccharide synthesis in one pot by selective coupling of isopropenyl and n-pentenyl glycopyranosides

HK Chenault, A Castro - Tetrahedron letters, 1994 - Elsevier
O-Isopropenyl glycosides bearing ester protecting groups are activated as glycosyl donors by
a variety of electrophiles. Diastereomerically pure β-glycoside products are formed in good …