Transcriptional control of preadipocyte determination by Zfp423

…, RJ Mepani, L Ye, HM Conroe, YA Roby, H Kulaga… - Nature, 2010 - nature.com
… e, Pparg2 mRNA levels in undifferentiated NIH 3T3 cells expressing a control vector or
full-length Zfp423 after 48 h of incubation with increasing doses of BMP4. f, Pparg2 gene …

Loss of BBS proteins causes anosmia in humans and defects in olfactory cilia structure and function in the mouse

HM Kulaga, CC Leitch, ER Eichers, JL Badano… - Nature …, 2004 - nature.com
… tissues with 30% sucrose plus 250 mM EDTA for 24 h at 4 C, froze them in OCT compound
(… We diluted the primary antibody in buffer T1 and incubated sections at 20 C for 1 h. We used …

Elevated ACE-2 expression in the olfactory neuroepithelium: implications for anosmia and upper respiratory SARS-CoV-2 entry and replication

M Chen, W Shen, NR Rowan, H Kulaga… - European …, 2020 - Eur Respiratory Soc
… We further quantified olfactory ACE-2 expression and found the number of ACE-2-positive
cells to be comparable between healthy controls and CRS (figure 1a, b and h), a common …

Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks

…, A Folkmann, DH Goldman, H Kulaga… - Proceedings of the …, 2017 - National Acad Sciences
The RNA-guided DNA endonuclease Cas9 has emerged as a powerful tool for genome
engineering. Cas9 creates targeted double-stranded breaks (DSBs) in the genome. Knockin of …

Loss of Bardet–Biedl syndrome protein-8 (BBS8) perturbs olfactory function, protein localization, and axon targeting

ALD Tadenev, HM Kulaga… - Proceedings of the …, 2011 - National Acad Sciences
Bardet–Biedl syndrome (BBS) is a pleiotropic, heterogeneous human disease whose etiology
lies primarily in dysfunctional basal bodies and/or cilia. Both BBS patients and several …

Recombinant antibodies: a new reagent for biological agent detection

…, JS Gebhardt, J Aldrich, EAE Garber, H Kulaga… - Biosensors and …, 2000 - Elsevier
… (PBS with 0.01% Tween-20) and blocked for 1 h at 37C with ELISA blocking buffer (5% Bacto
… as described above and incubated at 37C for 1 h. Botulinum toxin A or botulinum toxin B (…

Chronic interleukin‐13 expression in mouse olfactory mucosa results in regional aneuronal epithelium

A Saraswathula, MM Liu, H Kulaga… - International forum of …, 2023 - Wiley Online Library
… The whole nasal cavity with attached cribriform plate was dissected out and post-fixed in 4%
PFA on ice for 1 h with gentle rotation. After being washed in PBS, tissues were equilibrated …

Aerosol characteristics in a subway environment

…, S Francesconi, E Valdes, H Kulaga - Aerosol Science & …, 2003 - Taylor & Francis
<p>This paper presents an attempt to characterize biological and nonbiological aerosols in
a subway environment. This opportunity to study a subway station atmosphere was …

[HTML][HTML] Neuronal FcγRI mediates acute and chronic joint pain

…, SM Jeon, T Chen, Y Liu, H Kulaga… - The Journal of …, 2019 - Am Soc Clin Investig
… (H) Representative sections of knee joint taken 1 hour after ia injection with PBS, monomeric
IgG, or IgG-IC, stained with H&E, and scored for synovitis. S, synovium. Scale bar: 100 μm. …

Mouse urinary biomarkers provide signatures of maturation, diet, stress level, and diurnal rhythm

…, SJ Dixon, JE Zeskind, HM Kulaga… - Chemical …, 2010 - academic.oup.com
… Specifically, we identified volatile organic compounds (VOCs) in individual urine samples
taken from inbred C57BL/6J-H-2 b mice under several experimental conditions—maturation …