WAVE2 belongs to a family of proteins that mediates actin reorganization by relaying signals
from Rac to the Arp2/3 complex, resulting in lamellipodia protrusion. WAVE2 displays Arp2/…

Aurora family serine/threonine kinases control mitotic progression, and their deregulation is
implicated in tumorigenesis. Aurora A and Aurora B, the best-characterized members of …

CDK5 plays an indispensable role in the central nervous system, and its deregulation is
involved in neurodegeneration. We report the crystal structure of a complex between CDK5 and …

Protein ubiquitination is critical for numerous cellular functions, including DNA damage
response pathways [1, 2]. Histones are the most abundant monoubiquitin conjugates in …

Analysis of [3H]phorbol-12,13-dibutyrate (PDBu) binding was performed with protein kinase
C (PKC)-alpha, -beta 1, -gamma, -delta, -epsilon, -eta, and -zeta produced in Sf9 insect cells …

EBP1 was identified as a protein that interacts with the ErbB-3 receptor and possibly
contributes to transducing growth regulatory signals. The existence of EBP1 homologs across …

Genetic and biochemical evidence demonstrated that Eps8 is involved in the routing of signals
from Ras to Rac. This is achieved through the formation of a tricomplex consisting of Eps8…

… Classic “in-gel digestion” was performed according to the standard protocol [24], but in a
96-well plate for parallel processing (Areces and Rappsilber, manuscript in preparation). In brief…

Phosphorylation is one of the most frequently occurring post-translational modifications in
proteins. In eukaryotic cells, protein phosphorylation on serine, threonine and tyrosine …

Clathrin-mediated endocytosis (CME) involves spatially and temporally restricted molecular
dynamics, to which protein kinases and actin contribute. However, whether and how these …