Optimization of inclusion body solubilization and renaturation of recombinant human growth hormone from Escherichia coli
…, R Mukhija, A Krishnan, LC Garg… - Protein expression and …, 2000 - Elsevier
Recombinant human growth hormone (r-hGH) was expressed in Escherichia coli as
inclusion bodies. In 10 h of fed-batch fermentation, 1.6 g/L of r-hGH was produced at a cell …
inclusion bodies. In 10 h of fed-batch fermentation, 1.6 g/L of r-hGH was produced at a cell …
Toxin-antitoxin systems and their medical applications: current status and future perspective
Almost all bacteria synthesize two types of toxins—one for its survival by regulating different
cellular processes and another as a strategy to interact with host cells for pathogenesis. …
cellular processes and another as a strategy to interact with host cells for pathogenesis. …
Mineralocorticoid effects on Na-K-ATPase in individual nephron segments
LC Garg, MA Knepper, MB Burg - American Journal of …, 1981 - journals.physiology.org
A fluorometric micromethod for the assay of Na-K-ATPase was used to determine Na-K-ATPase
activity in 11 segments of the rabbit nephron. The Na-K-ATPase activity was found to be …
activity in 11 segments of the rabbit nephron. The Na-K-ATPase activity was found to be …
Solubilization of inclusion body proteins using n-propanol and its refolding into bioactive form
Inclusion bodies of recombinant human growth hormone (r-hGH) were isolated from Escherichia
coli, enriched and solubilized in 100mM Tris buffer containing 6M n-propanol and 2M …
coli, enriched and solubilized in 100mM Tris buffer containing 6M n-propanol and 2M …
Autocrine inhibition of Na+/K (+)-ATPase by nitric oxide in mouse proximal tubule epithelial cells.
…, SS Tang, JR Ingelfinger, LC Garg - The Journal of …, 1995 - Am Soc Clin Investig
… After incubating the cells in the reaction mixture for 30 min, fluorescence was measured and
Na/K ATPase activity determined using the corresponding formula as described by Garg (16)…
Na/K ATPase activity determined using the corresponding formula as described by Garg (16)…
Induced spawning of catfish, Heteropneustes fossilis (Bloch), using D-Lys6 salmon gonadotropin-releasing hormone analog
In this study, the effect of d-Lys 6 salmon gonadotropin-releasing hormone analog (sGnRH-A)
on spawning of Heteropneustes fossilis has been evaluated. A single intramuscular …
on spawning of Heteropneustes fossilis has been evaluated. A single intramuscular …
Immune responses and protective efficacy of recombinant outer membrane protein R (rOmpR)-based vaccine of Aeromonas hydrophila with a modified adjuvant …
Despite the importance and success of developing a candidate vaccine against Aeromonas
hydrophila infection in fish, little is known about the molecular mechanisms of the vaccine-…
hydrophila infection in fish, little is known about the molecular mechanisms of the vaccine-…
Development of a recombinant epsilon toxoid vaccine against enterotoxemia and its use as a combination vaccine with live attenuated sheep pox virus against …
…, GS Rani, SP Vijayan, D Mathur, LC Garg… - Clinical and Vaccine …, 2010 - Am Soc Microbiol
Sheep pox and enterotoxemia are important diseases of sheep, and these diseases cause
severe economic losses to sheep farmers. The present study was undertaken to evaluate the …
severe economic losses to sheep farmers. The present study was undertaken to evaluate the …
Role of DNA topoisomerase I in the transcription of supercoiled rRNA gene.
LC Garg, S DiAngelo, ST Jacob - Proceedings of the …, 1987 - National Acad Sciences
The fraction DE-B obtained by fractionating an extract from rat mammary adenocarcinoma
cells on a DEAE-Sephadex column was used for transcribing linear and supercoiled rRNA …
cells on a DEAE-Sephadex column was used for transcribing linear and supercoiled rRNA …
The rates of hydration of carbon dioxide and dehydration of carbonic acid at 37
LC Garg, TH Maren - Biochimica et Biophysica Acta (BBA)-General …, 1972 - Elsevier
The rates of hydration of CO 2 and dehydration of H 2 CO 3 were determined using a
stopped-flow spectrophotometric apparatus. The hydration rate constant, k 1 , was found to be …
stopped-flow spectrophotometric apparatus. The hydration rate constant, k 1 , was found to be …