… and dosed at 10 mg/kg body weight (10 mL/kg). To minimize variation in … /mL). This solution
was diluted with saline to 2 mg/mL and injected as single bolus at a dose of 10 mg/kg (5 mL/…

… The uptake was terminated after 1 min by washing the cells twice with 1 mL ice-cold PBS or
incubation medium, followed by lysis of cells in 0.5 mL 0.5 M NaOH. Subsequently, 0.4 mL of …

… Vlaming. A streamlined workflow for preclinical assessment of monoclonal antibody therapies:
A case study [abstract]. In: Proceedings of the American Association for Cancer Research …

… ml). This solution was diluted with saline to 2 mg/ml and injected as single bolus at a dose of
10 mg/kg (5 ml… and feces were homogenized in 4% BSA (1 ml per 100 mg feces). Part of the …

… twice with 1 mL ice-cold PBS or incubation medium, followed by the addition of 0.5 mL 0.5
M NaOH, of which 0.4 mL of the cell lysate was transferred to a 20 mL plastic scintillation vial …

… ml uridine diphosphoglucuronic acid, 10 μl of 0.5 mg/ml alamethicine and 10 μl of a pooled
human liver microsomal suspension (20 mg/ml… were pooled and diluted to 10 ml using 50% (v…

… For evaluation of radioactivity gels were sliced into 1-mm segments with a Hoefer gel slicer,
slices were dissolved at 50C in 0.3 ml H202-50/~1 NH4OH, and 5 ml of Aquasol was added …

… Teniposide (Vumon; 10 mg/mL) was 500-fold diluted with methanol to 20 μg/mL, and 100
μL of this solution were added as internal standard. Etoposide was extracted with 2 mL of 1,2-…

… 4 h after administration of morphine is approximately 1000 ng/ml. This means that the Mrp2
(-/-… This means that M3G concentration needs to remain on average 1000 ng/ml for 14 h (212 …

… ) were pumped through C 8 solid-phase-extraction columns (5-10 ml/min). The initial 200 ml
… liquid chromatography-grade methanol (3 ml at 1 ml/min) to sequentially remove nonpolar …