… HeLa cells were grown to confluence on sterilized glass coverslips in 35-mm culture
dishes in DMEM (with 44 mM NaHCO 3 , pH 7.2) supplemented with 10% vol/vol FBS, 100 U/ml …

Calmodulin (CaM) has been implicated in mediating the Ca 2+ -dependent regulation of gap
junctions. This report identifies a CaM-binding motif comprising residues 136–158 in the …

… mM EGTA was titrated by gradually adding 5–10 μl aliquots of the peptide stock solution (0.25–0.8
mM… μl aliquots of 50 or 15 mM Ca 2+ stock solution in Ca 2+ equilibrium buffer with 50 …

We have shown previously that the Ca 2+ -dependent inhibition of lens epithelial cell-to-cell
communication is mediated in part by the direct association of calmodulin (CaM) with …

… The protein was eluted from the column with a gradient of imidazole in 50 mM NaH 2 PO 4
/Na 2 HPO 4 and 250 mM NaCl (pH 7.4) and identified by mass spectrometry. Imidazole was …

… cells was placed in a 35-mm petri dish, sealed, flash frozen in … with 1 mm outside diameter,
0.75 mm inner diameter, and an … Micropipettes backfilled with a 1 mM solution of AF594 were …

… AChR-rich membranes were incubated for 1 h at ambient temperature in the presence of
500 nM CrV and 0.1 mM carbamylcholine in the presence or absence of 1 mM PCP. …

… Two milliliters of this cell suspension was placed into a 35-mm plastic petri plate containing
a 25-mm-diameter glass coverslip coated with poly-l-ornithine (100 μg/ml). Cells were grown …

… PCP was titrated into cuvettes containing 350 nM quinacrine in 10 mM phosphate buffer,
pH 7.4, with 1 mM carbamylcholine, and Torpedo AChR (0.75 mg/mL protein, ·), 0.75 mg/mL …

… measured in 300 mM NaCl and … mM Tb31-chelate in 10 mM HEPES, pH 7.0. Decay rates
for the chelate alone (black dots), with 2 mM CrV (blue dots), and with 2 mM CrV bound to 3 mM …