Thyroid-stimulating hormone and thyroid-stimulating hormone receptor structure-function relationships

MW Szkudlinski, V Fremont, C Ronin… - Physiological …, 2002 - journals.physiology.org
This review focuses on recent advances in the structure-function relationships of thyroid-stimulating
hormone (TSH) and its receptor. TSH is a member of the glycoprotein hormone …

Novel insights into the molecular mechanisms of human thyrotropin action: structural, physiological, and therapeutic implications for the glycoprotein hormone family

…, BD Weintraub, MW Szkudlinski - Endocrine …, 1997 - academic.oup.com
Mariusz W. Szkudlinski … Grossmann, MW Szkudlinski, and BD Weintraub, unpublished
data), development of suitable hTSH expression systems using eukaryotic cells (14, 50, 63, 64) …

The extracellular domain suppresses constitutive activity of the transmembrane domain of the human TSH receptor: implications for hormone-receptor interaction and …

…, D Puett, BD Weintraub, MW Szkudlinski - …, 2000 - academic.oup.com
… The oWT or mutant o6K cDNAs (residues M1-S116, where M1-S24 Address correspondence
to: Mariusz W. Szkudlinski, Laboratory of is the human o-subunit signal peptide) were first …

Autoantibody mimicry of hormone action at the thyrotropin receptor

…, AFM Pinto, JK Diedrich, Y Muftuoglu, MW Szkudlinski… - Nature, 2022 - nature.com
Thyroid hormones are vital in metabolism, growth and development 1 . Thyroid hormone
synthesis is controlled by thyrotropin (TSH), which acts at the thyrotropin receptor (TSHR) 2 . In …

Minireview: structural and functional evolution of the thyrotropin receptor

NR Farid, MW Szkudlinski - Endocrinology, 2004 - academic.oup.com
TSH receptor (TSHR) is a member of the leucine-rich repeat-containing G protein-coupled
receptors. Both TSHR and its ligand TSH have evolved to acquire specificity, minimize cross-…

Engineering human glycoprotein hormone superactive analogues

MW Szkudlinski, NG Teh, M Grossmann… - Nature …, 1996 - nature.com
We report the generation of superactive analogues of human glycoprotein hormones, with
potential applications in thyroid and reproductive disorders. Current biological and structural …

[HTML][HTML] Substitution of the seat-belt region of the thyroid-stimulating hormone (TSH) β-subunit with the corresponding regions of choriogonadotropin or follitropin …

M Grossmann, MW Szkudlinski, R Wong… - Journal of Biological …, 1997 - ASBMB
The region between the 10th and 12th cysteine (Cys 88 -Cys 105 in human thyroid-stimulating
hormone β-subunit (hTSHβ)) of the glycoprotein hormone β-subunits corresponds to the …

Asparagine-linked oligosaccharide structures determine clearance and organ distribution of pituitary and recombinant thyrotropin

MW Szkudlinski, NR Thotakura, JE Tropea… - …, 1995 - academic.oup.com
The recombinant human TSH (rhTSH) with highly sialylated oligosaccharide chains showed
higher in vivo bioactivity and a lower MCR than the predominantly sulfated pituitary human …

Recombinant thyrotropin containing a beta-subunit chimera with the human chorionic gonadotropin-beta carboxy-terminus is biologically active, with a prolonged …

…, Y Murata, FE Wondisford, MW Szkudlinski… - …, 1995 - academic.oup.com
Recombinant TSH is now successfully being used in clinical studies of thyroid cancer. Because
of its therapeutic potential, we have constructed a longer acting analog of TSH by fusing …

[HTML][HTML] Expression of human thyrotropin in cell lines with different glycosylation patterns combined with mutagenesis of specific glycosylation sites: Characterization …

M Grossmann, MW Szkudlinski, JE Tropea… - Journal of Biological …, 1995 - ASBMB
We used a novel approach to study the role of the Asn-linked oligosaccharides for human
thyrotropin (hTSH) activity. Mutagenesis of Asn(N) within individual glycosylation recognition …