[HTML][HTML] mTOR regulation of autophagy

CH Jung, SH Ro, J Cao, NM Otto, DH Kim - FEBS letters, 2010 - Elsevier
Nutrient starvation induces autophagy in eukaryotic cells through inhibition of TOR (target of
rapamycin), an evolutionarily-conserved protein kinase. TOR, as a central regulator of cell …

ULK-Atg13-FIP200 complexes mediate mTOR signaling to the autophagy machinery

…, CB Jun, SH Ro, YM Kim, NM Otto… - Molecular biology of …, 2009 - Am Soc Cell Biol
… Cells were then treated with rapamycin at 50 nM for 1 h in the presence of the isotope.
Myc-ULK1 or Atg13 immunoprecipitates were obtained by immunoprecipitation using anti-myc …

Regulatory cell therapy in kidney transplantation (The ONE Study): a harmonised design and analysis of seven non-randomised, single-arm, phase 1/2A trials

…, N Dupas, M Kapinsky, I Mutzbauer, NM Otto… - The Lancet, 2020 - thelancet.com
Background Use of cell-based medicinal products (CBMPs) represents a state-of-the-art
approach for reducing general immunosuppression in organ transplantation. We tested multiple …

The ULK1 complex mediates MTORC1 signaling to the autophagy initiation machinery via binding and phosphorylating ATG14

JM Park, CH Jung, M Seo, NM Otto, D Grunwald… - Autophagy, 2016 - Taylor & Francis
… HEK293T cells were incubated in HBSS medium deprived of amino acids or RPMI medium
deprived of glucose, or treated with rapamycin (100 nM) or Torin1 (250 nM) in full DMEM …

Regulatory T cells for minimising immune suppression in kidney transplantation: phase I/IIa clinical trial

A Roemhild, NM Otto, G Moll, M Abou-El-Enein… - bmj, 2020 - bmj.com
Objective To assess whether reshaping of the immune balance by infusion of autologous
natural regulatory T cells (nTregs) in patients after kidney transplantation is safe, feasible, and …

ULK1 inhibits the kinase activity of mTORC1 and cell proliferation

CH Jung, M Seo, NM Otto, DH Kim - Autophagy, 2011 - Taylor & Francis
… ULK1 MEFs were incubated in DMEM without serum overnight and treated with insulin (10
nM) for different periods of incubation time. The phosphorylation states of S6K1 (p-Thr389) …

ULK1 phosphorylates Ser30 of BECN1 in association with ATG14 to stimulate autophagy induction

…, M Seo, CH Jung, D Grunwald, M Stone, NM Otto… - Autophagy, 2018 - Taylor & Francis
… MEFs and HCT116 cells were treated with Torin 1 (250 nM) or rapamycin (100 nM) for
the periods of time as indicated. (C) Starvation-induced phosphorylation of BECN1 Ser30 …

GABARAPs and LC3s have opposite roles in regulating ULK1 for autophagy induction

DS Grunwald, NM Otto, JM Park, D Song, DH Kim - Autophagy, 2020 - Taylor & Francis
… WT, LC3 TKO, or GABARAP TKO HeLa cells were cultured in full medium or EBSS for 3 h
in the presence or absence of 100 nM bafilomycin A 1 (BAFA1). (I) Quantitative analysis of …

Preformed T cell alloimmunity and HLA eplet mismatch to guide immunosuppression minimization with tacrolimus monotherapy in kidney transplantation: Results of …

…, B Nashan, F Thaiss, NM Otto… - American Journal of …, 2021 - Wiley Online Library
Personalizing immunosuppression is a major objective in transplantation. Transplant
recipients are heterogeneous regarding their immunological memory and primary alloimmune …

Impact of acute kidney injury on neurological outcome and long-term survival after cardiac arrest–a 10 year observational follow up

…, M Engels, R Schindler, A Kahl, NM Otto - Journal of critical …, 2018 - Elsevier
Background Acute kidney injury (AKI) may be associated with short- and long-term patient
morbidity and mortality. Therefore, the impact of AKI after cardiac arrest on survival and …