The folding of proinsulin, the single-chain precursor of insulin, ensures native disulfide
pairing in pancreatic β-cells. Mutations that impair folding cause neonatal diabetes mellitus. …

We have recently developed a method to produce native human proinsulin using a bacterial
expression system. A proinsulin fusion protein was recovered from inclusion bodies and …

Insulin fibrillation provides a model for a broad class of amyloidogenic diseases. Conformational
distortion of the native monomer leads to aggregation-coupled misfolding. Whereas β-…

Second-generation antipsychotics are widely used to medicate patients with schizophrenia,
but may cause metabolic side effects such as diabetes, which has been considered to result …

The mouse tumor cell line αTC1-6 was used as a model system to examine the post-translational
processing of proglucagon. Determination of the mouse preproglucagon cDNA …

Protein evolution is constrained by folding efficiency ("foldability") and the implicit threat of
toxic misfolding. A model is provided by proinsulin, whose misfolding is associated with β-cell …

In familial hyperproinsulinemia, specific mutations in the proinsulin gene are linked with a
profound increase in circulating plasma proinsulin levels. However, the molecular and cellular …

In order to study whether hormone-sensitive tyrosine aminotransferase exists in tissues
other than liver, we have devised means to separate the liver-specific enzyme from other …

… Cell 37:1075-l 089 Mackin RB, Noe BD 1987 Direct evidence for two distinct … ME,
Eilertson CD, Klein K, Zhou Y, Lindberg I, McDonald JK, Mackin RB, Noe BD 1995 Processing …

… Mackin RB, Noe BD 1987 Direct evidence for two distinct prosomatostatin converting enzymes.
Detection using a rapid, sensitive, and specific assay for propeptide converting enzymes. …