The G-protein regulatory (GPR) motif in AGS3 was recently identified as a region for protein
binding to heterotrimeric G-protein α subunits. To define the properties of this ∼20-amino …

… Author links open overlay panel JL Boyer , SG Graber , GL Waldo , TK Harden , JC Garrison
… SG Graber and JC Garrison, unpublished observations. turkey erythrocyte enzyme (Fig. 2). …

The molecular basis of selectivity in G-protein receptor coupling has been explored by
comparing the abilities of G-protein heterotrimers containing chimeric Gα subunits, comprised of …

G protein-coupled receptors (GPCRs) are seven-transmembrane (TM) helical proteins that
bind extracellular molecules and transduce signals by coupling to heterotrimeric G proteins in …

The D 2 dopamine receptor has been expressed in Sf21 insect cells together with the G proteins
G o and G i2 , using the baculovirus system. Expression levels of receptor and G protein …

… Recombinant α i/o subunits were expressed in Sf9 cells and purified as described (Graber
et al., 1992a) except that the final chromatography step was performed on 15 μ Waters Protein…

The alpha subunits of the heterotrimeric guanine nucleotide-binding proteins Gi1, Gi2, Gi3,
G0, and Gs have been overexpressed in Sf9 cells using a baculovirus expression system. …

We previously reported that residues 299–318 in Gα i1 participate in the selective interaction
between Gα i1 and the 5-hydroxytryptamine 1B (5-HT 1B ) receptor (Bae, H., Anderson, K., …

The recognition between G protein and cognate receptor plays a key role in specific cellular
responses to environmental stimuli. Here we explore specificity in receptor-G protein …

The G-protein regulatory (GPR) motif, a conserved 25–30 amino acid domain found in multiple
mammalian proteins, stabilizes the GDP-bound conformation of Gα i , inhibits guanosine 5…