Autophagy is an evolutionary conserved catabolic process involved in several physiological
and pathological processes such as cancer and neurodegeneration. Autophagy initiation …

By crossing Huntington's disease (HD) R6/1 transgenic mice with ‘tissue’transglutaminase (TG2)
knock-out mice, we have demonstrated that this multifunctional enzyme plays an …

A sequential study of the appearance of liver cell death after thioacetamide (TH) administration
was performed in male Wistar rats. Within 3 hours of a single dose of TH, occurrence of …

… Note the intense staining in the colonies of rat hepatecytes interspersed among stromal
demen(s on their 4th day of primary culture. In the inset a phagosome containing an apoptotia …

The retinoblastoma gene product (pRB) plays an important role in controlling both cell release
from the G 1 phase and apoptosis. We show here that in the early phases of apoptosis, …

AIMS: Aloe-emodin (AE), a natural hydroxyanthraquinone compound, has been reported as
a potential anticancer agent. We studied the antineoplastic properties of AE on highly …

… Considering the role for TG2 in the mitochondrial homeostasis,12 and given the function of
2-DG in regulating cell’s ATP content, we decided to analyze 2-DG’s effect on autophagy in …

… Extracts were sonicated using the BioruptorH Twin (Diagenode) for two runs of ten cycles
(30 s on, 30 s off) at high-power setting. Cell lysate was centrifuged at 12,000g for 10 min at 4 C…

The phosphorylation of the serine 10 at histone H3 has been shown to be important for
transcriptional activation. Here, we report the molecular mechanism through which H3S10ph …

This unit provides two protocols for extraction of RNA from yeast that differ primarily in the
method for lysing the yeast cells. The first protocol isolates RNA directly from intact yeast cells …