Positive cooperativity of acetylcholine and other agonists with allosteric ligands on muscarinic acetylcholine receptors

J Jakubík, L Bačáková, EE El-Fakahany, S Tuček - Molecular pharmacology, 1997 - ASPET
… in plastic dishes without coating in Dulbecco’s modified Eagle’s medium with 10% fetal calf
… mild trypsinization, suspended in Dulbecco’s modified Eagle’s medium, and centrifuged for 3 …

Allosteric modulation of muscarinic acetylcholine receptors

S Tuček, J Proška - Trends in pharmacological sciences, 1995 - cell.com
… receptor ligands s. … s-8. A consistent feature of the action of gallamine is its ability to slow
down the dissociation of radiolabelled muscarinic receptor antagonists from their binding sites s,…

Choline acetyltransferase and the synthesis of acetylcholine

S Tuček - The cholinergic synapse, 1988 - Springer
Soon after the discovery that acetylcholine (ACh) mediates synaptic transmission in
sympathetic ganglia, Brown and Feldberg (1936) noted that the amount of ACh released from …

Quantitation of mRNAs for M1 to M5Subtypes of Muscarinic Receptors in Rat Heart and Brain Cortex

A Krejčı́, S Tuček - Molecular pharmacology, 2002 - ASPET
… The temperature profile was 15 min at 95C, 35 s at 60C, and 45 s at 72C, followed by cycles
of 45 s at 95C, 35 s at 62C, and 40 s at 72C, and finished with 7 min at 72C. The number of …

[HTML][HTML] Problems in the organization and control of acetylcholine synthesis in brain neurons

S Tuček - Progress in biophysics and molecular biology, 1984 - Elsevier
Acetylcholine (ACh) is the substance responsible, in the mammalian nervous system, for the
transfer of nerve-impulse-coded information from motor nerves to skeletal muscles, from …

The synthesis of acetylcholine in skeletal muscles of the rat

S Tuček - The Journal of physiology, 1982 - Wiley Online Library
… The incubation medium contained non-labelled acetyl-CoA instead of [14C-acetyl]CoA;
in some tubes, eserine was replaced by acetylcholinesterase (Sigma type VI S, 2 units per …

Provenance of the acetyl group of acetylcholine and compartmentation of acetyl‐CoA and Krebs cycle intermediates in the brain in vivo

S Tuček, SC Cheng - Journal of Neurochemistry, 1974 - Wiley Online Library
The origin of the acetyl group in acetyl‐CoA which is used for the synthesis of ACh in the
brain and the relationship of the cholinergic nerve endings to the biochemically defined …

Dual effects of muscarinic M2 acetylcholine receptors on the synthesis of cyclic AMP in CHO cells: dependence on time, receptor density and receptor agonists

P Michal, M Lysíková, S Tuček - British journal of pharmacology, 2001 - Wiley Online Library
… We propose that the G s -mediated stimulatory component of … s proteins. It tends to be
abolished by receptor internalization. … s or G i will be reflected by differences in the ratios of EC 50-…

Management of pheochromocytomas and paragangliomas: Review of current diagnosis and treatment options

M Eid, J Foukal, D Sochorová, Š Tuček… - Cancer …, 2023 - Wiley Online Library
… Intraoperative ultrasound can optimize tumor localization in both tissue- s parring techniques
as well as standard adrenalectomies. Surgical resection also remains the main treatment …

Utilization of citrate, acetylcarnitine, acetate, pyruvate and glucose for the synthesis of acetylcholine in rat brain slices

V Doležal, S Tuček - Journal of neurochemistry, 1981 - Wiley Online Library
… were then extracted into 1.0 ml of a solution of TPB in butyl acetate (15 mg/ml); the radioactivity
in the TPBibutyl acetate extract was measured by liquid scintillation spectrometry (TuCek, …