T-type calcium channel blockers that attenuate thalamic burst firing and suppress absence seizures

…, J Mezeyova, M Weerapura, C Eduljee… - Science translational …, 2012 - science.org
Absence seizures are a common seizure type in children with genetic generalized epilepsy
and are characterized by a temporary loss of awareness, arrest of physical activity, and …

Analgesic effects of a substituted N-triazole oxindole (TROX-1), a state-dependent, voltage-gated calcium channel 2 blocker

…, VA Warren, B Williams, SP Arneric, C Eduljee… - … of Pharmacology and …, 2010 - ASPET
C, followed by 0.05% trypsin for 10 min at 37C. The ganglia were then washed in F-14 growth
media, consisting of 10% F-14, 10% horse serum, 1% penicillin/streptomycin (5000 IU/500 …

Characterization of the substituted N-triazole oxindole TROX-1, a small-molecule, state-dependent inhibitor of Cav2 calcium channels

…, MHM Smith, VA Warren, SP Arneric, C Eduljee… - Molecular …, 2012 - ASPET
… at 30C for Ca V 2.2 and Ca V 2.3 cell lines and at 30C for 2 … Cells were incubated in the dark
at 25C for 60 to 70 min. Dye … incubated in the dark at 25C for 30 min. Fluorescence intensity …

A novel slow-inactivation-specific ion channel modulator attenuates neuropathic pain

ME Hildebrand, PL Smith, C Bladen, C Eduljee, JY Xie… - PAIN®, 2011 - Elsevier
… Ganglia were incubated for 45 min at 37 C and subsequently placed in L-15 media …
Coverslips were incubated at 37 C for 1–2 h and transferred to 4 C for storage. Within 72 h, …

Molecular determinants of the inhibition of human Kv1.5 potassium currents by external protons and Zn2+

SJ Kehl, C Eduljee, DCH Kwan… - The Journal of …, 2002 - Wiley Online Library
Using human Kv1.5 channels expressed in HEK293 cells we assessed the ability of H + o to
mimic the previously reported action of Zn 2+ to inhibit macroscopic hKv1.5 currents, and …

A fluorescence-based high-throughput screening assay for the identification of T-type calcium channel blockers

F Belardetti, E Tringham, C Eduljee, X Jiang… - Assay and drug …, 2009 - liebertpub.com
… The cell plates were incubated in a 5% CO2 humidified incubator at 37C and 1 day before
conducting the assay were shifted to an incubator at 29C in 5% CO2. On the day of the assay, …

Synergistic inhibition of the maximum conductance of Kv1.5 channels by extracellular K+ reduction and acidification

D Fedida, S Zhang, DCH Kwan, C Eduljee… - Cell biochemistry and …, 2005 - Springer
… Here we discuss P/C type inactivation as the common pathway by which some Kv … P-type,
C-type, and U-type inactivation is not known. Consequently, we prefer here to use the term P/C

SCAM analysis reveals a discrete region of the pore turret that modulates slow inactivation in Kv1. 5

C Eduljee, TW Claydon… - … of Physiology-Cell …, 2007 - journals.physiology.org
In Kv1.5, protonation of histidine 463 in the S5-P linker (turret) increases the rate of
depolarization-induced inactivation and decreases the peak current amplitude. In this study, we …

Constitutive inactivation of the hKv1.5 mutant channel, H463G, in K+-free solutions at physiological pH

S Zhang, C Eduljee, DCH Kwan, SJ Kehl… - Cell biochemistry and …, 2005 - Springer
… It is suggested that the histidine somehow regulates the function of the C-type inactivation …
penicillinstreptomycin, and 0.5 mg/mL geneticin at 37C in an atmosphere of 5% CO2 in air. All …

[PDF][PDF] The external K+ concentration and mutations in the outer pore mouth affect the inhibition of Kv1. 5 current by Ni2+

DCH Kwan, C Eduljee, L Lee, S Zhang, D Fedida… - Biophysical journal, 2004 - cell.com
… The term C-type inactivation was coined to describe the slow inactivation process in Shaker
that was … C-type inactivation is coupled to channel activation and is believed to involve a …