New colorimetric cytotoxicity assay for anticancer-drug screening
P Skehan, R Storeng, D Scudiero… - JNCI: Journal of the …, 1990 - academic.oup.com
We have developed a rapid, sensitive, and inexpensive method for measuring the cellular
protein content of adherent and suspension cultures in 96-well microtiter plates. The method …
protein content of adherent and suspension cultures in 96-well microtiter plates. The method …
Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines
A Monks, D Scudiero, P Skehan… - JNCI: Journal of the …, 1991 - academic.oup.com
We describe here the development and implementation of a pilot-scale, in vitro, anticancer
drug screen utilizing a panel of 60 human tumor cell lines organized into subpanels …
drug screen utilizing a panel of 60 human tumor cell lines organized into subpanels …
Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay
MC Alley, DA Scudiero, A Monks, ML Hursey… - Cancer research, 1988 - AACR
For the past 30 years strategies for the preclinical discovery and development of potential
anticancer agents have been based largely upon the testing of agents in mice bearing …
anticancer agents have been based largely upon the testing of agents in mice bearing …
Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines
DA Scudiero, RH Shoemaker, KD Paull, A Monks… - Cancer research, 1988 - AACR
We have previously described the application of an automated microculture tetrazolium
assay (MTA) involving dimethyl sulfoxide solubilization of cellular-generated 3-(4,5-…
assay (MTA) involving dimethyl sulfoxide solubilization of cellular-generated 3-(4,5-…
A gene expression database for the molecular pharmacology of cancer
…, WC Reinhold, TG Myers, DT Andrews, DA Scudiero… - Nature …, 2000 - nature.com
We used cDNA microarrays to assess gene expression profiles in 60 human cancer cell
lines used in a drug discovery screen by the National Cancer Institute. Using these data, we …
lines used in a drug discovery screen by the National Cancer Institute. Using these data, we …
IAP-family protein survivin inhibits caspase activity and apoptosis induced by Fas (CD95), Bax, caspases, and anticancer drugs
I Tamm, Y Wang, ED Sausville, DA Scudiero, N Vigna… - Cancer research, 1998 - AACR
Survivin is a member of the inhibitor of apoptosis protein (IAP) family. We investigated the
antiapoptotic mechanism of Survivin, as well as its expression in 60 human tumor cell lines …
antiapoptotic mechanism of Survivin, as well as its expression in 60 human tumor cell lines …
An information-intensive approach to the molecular pharmacology of cancer
…, WW Van Osdol, AP Monks, DA Scudiero… - Science, 1997 - science.org
Since 1990, the National Cancer Institute (NCI) has screened more than 60,000 compounds
against a panel of 60 human cancer cell lines. The 50-percent growth-inhibitory …
against a panel of 60 human cancer cell lines. The 50-percent growth-inhibitory …
Tetrazolium-based assays for cellular viability: a critical examination of selected parameters affecting formazan production
DT Vistica, P Skehan, D Scudiero, A Monks, A Pittman… - Cancer research, 1991 - AACR
… A decrease in the concentration of D-glucose from culture medium was accompanied by a …
D-glucose exhibited the greatest reduction in MTT specific activity. Further evi dence that the D…
D-glucose exhibited the greatest reduction in MTT specific activity. Further evi dence that the D…
Display and analysis of patterns of differential activity of drugs against human tumor cell lines: development of mean graph and COMPARE algorithm
…, L Hodes, A Monks, DA Scudiero… - JNCI: Journal of the …, 1989 - academic.oup.com
The objective of this study was to develop and investigate an approach to optimally detect,
rank, display, and analyze patterns of differential growth inhibition among cultured cell lines. …
rank, display, and analyze patterns of differential growth inhibition among cultured cell lines. …
Comparison of in vitro anticancer-drug-screening data generated with a tetrazolium assay versus a protein assay against a diverse panel of human tumor cell lines
…, S Tosini, P Skehan, DA Scudiero… - JNCI: Journal of the …, 1990 - academic.oup.com
The National Cancer Institute (NCI) is implementing a large-scale in vitro drug-screening
program that requires a very efficient automated assay of drug effects on tumor cell viability or …
program that requires a very efficient automated assay of drug effects on tumor cell viability or …