Here we describe a de novo tRNA acylation system, the flexizyme (Fx) system, for the
preparation of acyl tRNAs with nearly unlimited selection of amino and hydroxy acids and tRNAs. …

… We are thankful to our current and past co-workers at the Suga laboratory for creating an …
The body of the works discussed in this article from the Suga laboratory was supported by …

Conspectus Although macrocyclic peptides bearing exotic building blocks have proven their
utility as pharmaceuticals, the sources of macrocyclic peptide drugs have been largely …

We synthesized a library of Pseudomonas aeruginosa autoinducer analogs with variation
targeted to the homoserine lactone (HSL) moiety and discovered a new agonist, 3-oxo-C 12 -(2…

Naturally occurring peptides often possess macrocyclic and N-methylated backbone. These
features grant them structural rigidity, high affinity to targets, proteolytic resistance, and …

Macrocyclic peptides are an emerging class of therapeutics that can modulate protein–protein
interactions. In contrast to the heavily automated high-throughput screening systems …

… group of the ester bond in HSL accepts an H-bond from the indole NH group of Trp57, … an
H-bond. Thus the OH and keto group of 4 and 5, respectively, can mimic the ketone of HSL as H…

Genetic code reprogramming is a method for the reassignment of arbitrary codons from
proteinogenic amino acids to nonproteinogenic ones; thus, specific sequences of nonstandard …

… export xenobiotics using an electrochemical gradient of H + or Na + across the membrane 1
, … Here we present the crystal structures of the H + -driven MATE transporter from Pyrococcus …

The initiation codon dictates that the translation initiation event exclusively begins with
methionine. We report here a new technology to reprogram the initiation event, where various …