Quantification of functional selectivity at the human α1A-adrenoceptor

BA Evans, N Broxton, J Merlin, M Sato… - Molecular …, 2011 - ASPET
… normalized to that of norepinephrine (logTR n ), and the final bias factor calculations for
signaling by the α 1A -AR (difference between logTR n values for a given agonist across different …

[HTML][HTML] α-Conotoxin EpI, a Novel Sulfated Peptide from Conus episcopatusThat Selectively Targets Neuronal Nicotinic Acetylcholine Receptors

…, A Atkins, J Cuevas, DJ Adams, NM Broxton… - Journal of Biological …, 1998 - ASBMB
We have isolated and characterized α-conotoxin EpI, a novel sulfated peptide from the
venom of the molluscivorous snail, Conus episcopatus. The peptide was classified as an α-…

[HTML][HTML] SOD enzymes and microbial pathogens: surviving the oxidative storm of infection

CN Broxton, VC Culotta - PLoS pathogens, 2016 - journals.plos.org
Since oxygen appeared in the biosphere some 3–5 billion years ago, all organisms have
had to deal with the hazards of potentially damaging reactive oxygen species (ROS), such as …

Factors influencing biased agonism in recombinant cells expressing the human α1A‐adrenoceptor

…, M Sato, J Merlin, N Broxton… - British journal of …, 2017 - Wiley Online Library
… of interest to yield LogTR n in order to … n of one pathway from the other to give a ΔLogTR n
value, a numerical measure of bias. A lack of biased agonism will result in values of ΔLogTR n

α‐Conotoxin ImI inhibits the α‐bungarotoxin‐resistant nicotinic response in bovine adrenal chromaffin cells

NM Broxton, JG Down, J Gehrmann… - Journal of …, 1999 - Wiley Online Library
The activity of α‐conotoxin (α‐CTX), ImI, from the vermivorous marine snail Conus imperialis,
has been studied on mammalian nicotinic receptors on bovine chromaffin cells and at the …

THE JANUS* FACES OF ADRENOCEPTORS: FACTORS CONTROLLING THE COUPLING OF ADRENOCEPTORS TO MULTIPLE SIGNAL TRANSDUCTION …

RJ Summers, N Broxton… - Clinical and …, 2004 - Wiley Online Library
The adrenoceptors (AR) are an important subfamily of rhodopsin‐like G‐protein‐coupled
receptors that couple to an increasingly large number of signalling mechanisms. Two important …

[HTML][HTML] An Adaptation to Low Copper in Candida albicans Involving SOD Enzymes and the Alternative Oxidase

CN Broxton, VC Culotta - PloS one, 2016 - journals.plos.org
In eukaryotes, the Cu/Zn superoxide dismutase (SOD1) is a major cytosolic cuproprotein with
a small fraction residing in the mitochondrial intermembrane space (IMS) to protect against …

Optimizing the connectivity in disulfide-rich peptides: α-conotoxin SII as a case study

JP Bingham, NM Broxton, BG Livett, JG Down… - Analytical …, 2005 - Elsevier
We describe a strategy for the efficient, unambiguous assignment of disulfide connectivities
in α-conotoxin SII, of which ∼30% of its mass is cysteine, as an example of a generalizable …

Leu10 of α-conotoxin PnIB confers potency for neuronal nicotinic responses in bovine chromaffin cells

N Broxton, L Miranda, J Gehrmann, J Down… - European journal of …, 2000 - Elsevier
… It is probable that α-conotoxin EpI owes its enhanced activity in our assay to a greater
involvement of the N-terminal loop at the receptor than is the case with the other six peptides, in …

[HTML][HTML] Dichloroacetate and thiamine improve survival and mitochondrial stress in a C. elegans model of dihydrolipoamide dehydrogenase deficiency

CN Broxton, P Kaur, M Lavorato, S Ganesh, R Xiao… - JCI insight, 2022 - ncbi.nlm.nih.gov
Dihydrolipoamide dehydrogenase (DLD) deficiency is a recessive mitochondrial disorder
caused by depletion of DLD from α-ketoacid dehydrogenase complexes. Caenorhabditis …