… In particular, CXCL12 expression is increased by hypoxia-inducible factor (HIF)-1α, a
protein that regulates the transcription of over 60 genes involved in the tissue’s response to …

… An alternative strategy to perturb DNA methylation is based on S-adenosylhomocysteine
hydrolase as the target enzyme. S-Adenosylhomocysteine is a competitive inhibitor of S-…

Roughly 50% of cancer cases occur in people aged 65 years or older. Older people are often
diagnosed at a later stage and might receive less (intensive) treatment, which might affect …

… In polarized Madin–Darby canine kidney II (MDCKII) cells transfected with an MRP5
cDNA construct, MRP5 is routed to the basolateral membrane and these cells transport S-(2,4-…

This study was undertaken to demonstrate the unique specificity of the chemokine receptor
CXCR4 antagonist AMD3100. Calcium flux assays with selected chemokine/cell …

… of 30 s at 95C, 30 s at 64C, and 30 s at 72C, with a final extension step of 5 min at 72C.
Equal amounts of PCR products (for β 2 m and SDF-1) of the samples were analyzed on 1.5% …

… We generated tumor-conditioned medium (TCM) by culturing 4T1 primary breast tumors
in Dulbecco’s modified Eagle medium (DMEM) without fetal bovine serum (FBS) 11,12,13,14 …

… Robust biomarkers that reflect the body’s biological age can be helpful to provide older
patients with cancer with an optimal personalized treatment. However, to be able to identify such …

… Each cycle comprised a 45-s denaturation step at 95C, a 45-s annealing step at 61C, and
a 45-s extension step at 72C. In preliminary experiments, the exponential range of the PCR …

We correlated serum 25-hydroxyvitamin D 3 (25OHD) levels with tumor characteristics and
clinical disease outcome in breast cancer patients and assessed the impact of genetic …