Histone deacetylase 3 is required for centromeric H3K4 deacetylation and sister chromatid cohesion

Grégory Eot-Houllier; Géraldine Fulcrand; Yoshinori Watanabe; Laura Magnaghi-Jaulin; Christian Jaulin

doi:10.1101/gad.484108

Histone deacetylase 3 is required for centromeric H3K4 deacetylation and sister chromatid cohesion

¹ INSERM, U860, Montpellier, F-34298 France;
² CRLC Val d’Aurelle-Paul Lamarque, Montpellier, F-34298 France;
³ Université Montpellier1, Montpellier, F-34298 France;
⁴ Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113-0032, Japan.

Abstract

We describe here the role of histone deacetylase 3 (HDAC3) in sister chromatid cohesion and the deacetylation of histone H3 Lys 4 (H3K4) at the centromere. HDAC3 knockdown induced spindle assembly checkpoint activation and sister chromatid dissociation. The depletion of Polo-like kinase 1 (Plk1) or Aurora B restored cohesion in HDAC3-depleted cells. HDAC3 was also required for Shugoshin localization at centromeres. Finally, we show that HDAC3 depletion results in the acetylation of centromeric H3K4, correlated with a loss of dimethylation at the same position. These findings provide a functional link between sister chromatid cohesion and the mitotic “histone code”.

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Footnotes

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↵5 Present addresses: Groupe Microtubules et Cycle Cellulaire, Institut de Génétique Humaine, CNRS UPR 1142, rue de la cardonille, 34396 Montpellier cedex 5, France;
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↵6 CNRS UMR 6061, Institut de Génétique et Développement, Université de Rennes I, IFR 140, 2 Avenue du Pr Léon Bernard, 35043 Rennes cedex, France.
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↵7 Corresponding author.

↵7 E-MAIL christian.jaulin@univ-rennes1.fr; FAX 33-2-23-23-44-78.
Supplemental material is available at http://www.genesdev.org.
Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.484108.
- Received April 21, 2008.
- Accepted August 11, 2008.