Data Supplement
- Supplemental Data -
Supplemental Table 1 - Effect of RGS2 WT and mutant on AT1 receptor-mediated intracellular calcium release demonstrated by Max and EC50 derived AngII concentration response curves (* p< 0.05, *** p<0.001, compared to Ctrl; # p<0.05 compared to RGS2 WT)
Supplemental Figure 1 - MBP-RGS2 protein purification
Supplemental Figure 2 - Increasing amount of RGS2-V5 increases suppression of AT1R-mediated Ca2+ mobilization
Supplemental Figure 3 - Co-expression with RGS2 WT or mutants does not change HA-AT1R protein expression
Supplemental Figure 4 - Protein half-live of the Q2L and R188H
Supplemental Figure 5 - Initial fluorescent intensity of RGS2 WT, R188H and Q196R in DSF analysis
Supplemental Figure 6 - Effect of proteasomal inhibition on expression of R188H
SUPPLEMENTAL REFERENCES