Data Supplement
- Supplemental Data -
Supplemental Figure 1 - Relative levels of RAMX3 bound to membranes prepared from HEK293T cells transiently transfected with WT or CXCR3 receptor mutants
Supplemental Figure 2 - Dose-response curves showing that the negative allosteric modulators are inverse agonists at CXCR3 mutants
Supplemental Table 1 - Effects of mutation of residues in CXCR3 on protein expression[a] and affinities of negative allosteric modulators as determined by allosteric radioligand RAMX3 displacement assay[b]
Supplemental Table 2 - Effects of mutations on CXCL11- and CXCL10-mediated G protein activation as determined by cAMP BRET [a]
Supplemental Table 3 - Effects of mutations on CXCL11- and CXCL10-mediated β-Arrestin 1 recruitment as determined by β-Arrestin BRET assay [a]
Supplemental Table 4 - Effects of mutations on CXCL11- and CXCL10-mediated β-Arrestin 2 recruitment as determined by β-Arrestin BRET assay [a]
Supplemental Table 5 - Ability of allosteric modulators to inhibit the CXCL11-mediated G protein activation of the CXCR3 Wild Type or its mutants as determined by BRET [a]
Supplemental Table 6 - Ability of allosteric modulators to inhibit the CXCL10-mediated G protein activation of the CXCR3 Wild Type or its mutants as determined by BRET [a]
Supplemental Table 7 - Ability of allosteric modulators to inhibit the CXCL11-mediated β-Arrestin 1 recruitment of the CXCR3 Wild Type or its mutants as determined by BRET [a]
Supplemental Table 8 - Ability of allosteric modulators to inhibit the CXCL10-mediated β-Arrestin 1 recruitment of the CXCR3 Wild Type or its mutants as determined by BRET [a]
Supplemental Table 9 - Ability of allosteric modulators to inhibit the CXCL11-mediated β-Arrestin 2 recruitment of the CXCR3 Wild Type or its mutants as determined by BRET [a]
Supplemental Table 10 - Ability of allosteric modulators to inhibit the CXCL10-mediated β-Arrestin 2 recruitment of the CXCR3 Wild Type or its mutants as determined by BRET [a]