Data Supplement
Data Supplement
- Supplemental Data -
Supplemental Table 1 - Comparison of effects of mutations in rOCT1 on IC50 values determined for inhibition of MPP+ uptake by TEA+ with apparent Km values of TEA+ uptake
Supplemental Figure 1 - Equations which were applied for calculation of apparent Km values from substrate replacement curves (Figs. 3, 4, Supplemental Fig. 2) and of IC50 values from inhibition curves (Figs. 2, 5, 6, 7, Supplemental Figs. 3, 4)
Supplemental Figure 2 - Determination of apparent Km values for uptake of MPP+ and TEA+ in confluent HEK293, which were stably transfected with wildtype rOCT1 or rOCT1 mutants
Supplemental Figure 3 - Determination of IC50 values for inhibition of uptake of 0.1 μM MPP+ by TEA+ or TBuA+ in confluent HEK293, which stably expressed wildtype rOCT1 or mutants thereof
Supplemental Figure 4 - Impact of mutations in rOCT1 on inhibition of initial uptake of 0.1 μM MPP+ by TEA+ measured in dissociated HEK293 cells
Supplemental Figure 5 - Amino acid sequence alignment of rOCT1 (uniprot code Q63089) and hOCT1 (uniprot code O15245)
Supplementary Figure 6 - Cartoon representation of 3D homology models of rOCT1 and hOCT1 in the presumed outward-open conformations modeled by different approaches as described earlier (Gorbunov et al., 2008; Dakal et al., 2017)
Supplementary Figure 7 - Time courses of MPP+ uptake into confluent monolayers of HEK293 cells stably transfected with rOCT1, hOCT1 or control plasmid pcDNA5.1 that were grown on plastic dishes