Data Supplement
- Supplemental Data -
Supplemental Table 1 - Peptide sequences
Supplemental Table 2 - Statistical analysis of pKI values for AM/CGRP variants determined by FP assay
Supplemental Table 3 - Statistical analysis of apparent pKB values for AM/CGRP antagonism at RAMP:CLR receptors
Supplemental Table 4 X-ray diffraction data collection and refinement statistics
Supplemental Figure 1 - Development of a fluorescence polarization/anisotropy (FP) peptide-binding assay
Supplemental Figure 2 - Competition binding FP assay with AM and CGRP peptides obtained using purified MBP-RAMP1/2-CLR ECD fusion proteins
Supplemental Figure 3 - RAMP-altered CLR conformations in peptide-bound and ligand-free RAMPCLR ECD crystal structures
Supplemental Figure 4 - Scatter plots summarizing the pKI values for AM and CGRP variants obtained in the FP assay with purified MBP-RAMP1/2-CLR ECD fusion proteins
Supplemental Figure 5 - Antagonism of AM variants in cell-based signaling assays using COS-7 cells
Supplemental Figure 6 - Antagonism of CGRP variants in cell-based signaling assays using COS-7 cells
Supplemental Figure 7 - Scatter plots summarizing apparent pKB values of AM and CGRP variants obtained in the cell-based signaling assay
Supplemental Figure 8 - Crystal structure of an AMmut-bound MBP-RAMP1-CLR ECD complex
Supplemental Figure 9 - Cross-species amino acid sequence alignment of AM and CGRP
Supplemental Figure 10 - Comparison of RAMP3-CLR model to peptide-bound RAMP1- and RAMP2-
CLR ECD crystal structures - Supplemental Move -
RAMP-mediated conformational changes in the CLR ECD