Data Supplement
- Supplemental Data -
Supplemental Methods
Supplementary Table 1 – Summary of potency and maximum response parameters for VU0424465-mediated iCa2+ mobilization in HEK293A-mGlu5-low cells after 1 min pre-incubation with vehicle or class C GPCR allosteric modulators
Supplementary Table 2 – Summary of potency and maximum response parameters for ATP and CChmediated iCa2+ mobilization in non-transfected HEK293A cells after 30 min pre-incubation with vehicle or class C GPCR allosteric modulators
Supplementary Figure 1 - Effect of Class C GPCR allosteric ligands on [3H]methoxy-PEPy binding to HEK293A-mGlu5-low cells
Supplementary Figure 2 - Orthosteric agonist concentration response curves in the absence and presence of increasing concentrations of (A) BINA at mGlu2, (B) PHCCC at mGlu4, (C) CGP7930 and (D) GS39783 at GABAB, (E) ML337 at mGlu3, (F) AZ01226052 at mGlu8 and (G) VU0483605 at mGlu1
Supplementary Figure 3 - Class C GPCR allosteric ligands have no intrinsic efficacy for iCa2+ mobilization in HEK293A-mGlu5-low cells
Supplementary Figure 4 - Effect of diverse mGlu5 allosteric modulators on (A) extracellular Ca2+ (Ca2+o) and (B) AC265347 concentration response-curves for iCa2+ mobilization in Flp-In TREx HEK cells expressing CaSR
Supplementary Figure 5 - Modulation of CCh (A) and ATP (B) concentration response-curves for iCa2+ mobilization in non-transfected HEK293A cells by Class C GPCR allosteric modulators
Supplementary references