Data Supplement
- Supplemental Data -
Supplemental Table 1 - Determinations of GFP-transporter proteins that were incorporated into nanodiscs formed from MSP1E3D1 and different lipids
Supplemental Figure 1 - Evaluation of the employed experimental conditions for measuring MPP+ binding to GFP-rOCT1 on filters
Supplemental Figure 2 - Densitometric quantification of GFP-rOCT1 and MSP1E3D1 in SDS polyacrylamide gels
Supplemental Figure 3 - Reconstitution of GFP-rOCT1 into nanodiscs formed with MSP1E3D1 and POPC and their isolation
Supplemental Figure 4 - Binding of MPP+ to GFP-rOCT1 reconstituted into nanodiscs formed with POPC and MSP1E3D1 in the presence of 1 mM TBuA+