Supplemental Data
- Supplemental Figures -
Supplemental Figure 1: Scheme showing the synthesis of biotinylated metformin.
Supplemental Figure 2. Metformin protects against hyperglycaemia-induced increases in reactive oxygen species in murine microvascular endothelial cells.
Supplemental Figure 3: Metformin protects mitochondrial respiration from hyperglycaemia in wild-type but not Nr4a1-null aorta tissues.
Supplemental figure 4: Metformin improves extracellular acidification rate (ECAR), an indicator of glycolysis in wild-type endothelial cells.
Supplemental figure 5: Lack of effect of metformin to upregulate Nr4a1 mRNA in intact hyperglycaemia-exposed aorta rings and lack of effect of actinomycin D to prevent metformin from improving hyperglycaemia-impaired vascular vasodilator function in vitro.
Supplemental figure 6: In silico predicted interactions of cytosporone B and celastrol with NR4A1.
Supplemental figure 7: In silico predicted interactions of THPN with NR4A1.
Supplemental figure 8: In silico predicted interactions of TMPA with NR4A1.