Abstract
Phosphofructokinase from the guinea pig heart is an extremely labile enzynme. It becomes more stable in the presence of perchloric acid extracts from the liver. Fructose-1,6-P2 has been identified in these extracts as a potent stabilizer for the enzyme. The stabilizing effects for enzyme activity of the following four groups of compounds were tested: thiol derivatives, hexose phosphate, adenylic nucleotides, and polyvalent inorganic anions. The most potent representatives of these groups are: glutathione or mercaptoethanol, fructose-1,6-P2, ADP or ATP, and phosphate or sulfate anion. A combination of these agents, besides providing complete protection for the enzyme, causes a slight activation during incubation.
ACKNOWLEDGMENT Supported by Research Grant AI04214 from the National Institute of Allergy and Infectious Diseases, U.S. Public Health Service; Research Career Development Award GM-K3-3848 from the Division of General Medical Sciences, U.S. Public Health Service: and a grant from the Santa Clara County Heart Association.
- Copyright ©, 1965, by Academic Press Inc.
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