Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

Misreading of RNA Codewords Induced by Aminoglycoside Antibiotics

JULIAN DAVIES, LUIGI GORINI and BERNARD D. DAVIS
Molecular Pharmacology July 1965, 1 (1) 93-106;
JULIAN DAVIES
Department of Bacteriology and Immunology, Harvard Medical School, Boston, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
LUIGI GORINI
Department of Bacteriology and Immunology, Harvard Medical School, Boston, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
BERNARD D. DAVIS
Department of Bacteriology and Immunology, Harvard Medical School, Boston, Massachusetts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

The aminoglycoside antibiotics streptomycin, neomycin, kanamycin, paromomycin, gentamicin, hygromycin B, and viomycin all disturb the fidelity of the reading of polyribonucleotides in polypeptide synthesis. This effect is seen with all four homopolymers: poly U, poly C, poly A, and poly I.

The extent and the spectrum of misreading vary with the antibiotic used and with the sRNA concentration. The antibiotics also influence the incorporation of the "correct" amino acid: with poly A they cause either stimulation or inhibition of lysine incorporation, depending on the sRNA concentration; poly C-directed proline incorporation was stimulated at all sRNA concentrations tested; and with poly U-directed phenylalanine incorporation the drugs were only inhibitory with most extracts. In all systems, with increasing sRNA the inhibition by the drug of "correct" reading became more prominent, and the relative misreading less prominent.

In the light of recent knowledge of coding triplets, the effects observed suggest that aminoglycosides can (a) cause misreading of only one base at a time in UUU and CCC triplets, but of all three in AAA or III; (b) cause misreading of a base in only the 5' and the internal position in the pyrimidine codons, but in all three positions in the purine codons; and (c) allow both transition misreadings (purine to purine, or pyrimidine to pyrimidine) and transversion misreadings (purine to pyrimidine, or pyrimidine to purine). Transversion misreadings would seem to be rare in pyrimidine-containing polymers.

ACKNOWLEDGMENTS We wish to thank Susanne Armour for her patient and skillful technical assistance. Dr. Martin Lubin made valuable comments during the preparation of this manuscript. Aided by grants from the National Science Foundation (GB-1307), U.S. Public Health Service (AI-02011-08), and Bristol Laboratories.

  • Copyright ©, 1965, by Academic Press Inc.

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 1, Issue 1
1 Jul 1965
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Misreading of RNA Codewords Induced by Aminoglycoside Antibiotics
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Misreading of RNA Codewords Induced by Aminoglycoside Antibiotics

JULIAN DAVIES, LUIGI GORINI and BERNARD D. DAVIS
Molecular Pharmacology July 1, 1965, 1 (1) 93-106;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Research ArticleArticle

Misreading of RNA Codewords Induced by Aminoglycoside Antibiotics

JULIAN DAVIES, LUIGI GORINI and BERNARD D. DAVIS
Molecular Pharmacology July 1, 1965, 1 (1) 93-106;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Nelfinavir and PXR
  • P2X7 Positive Modulator Structure-Activity Relationship
  • Predicting Drug Interactions with ENT1 and ENT2
Show more Articles

Similar Articles

  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2021 by the American Society for Pharmacology and Experimental Therapeutics