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Molecular Pharmacology

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Research ArticleArticle

Inhibitor Binding Analysis of Dihydrofolate Reductases from Various Species

JAMES J. BURCHALL and GEORGE H. HITCHINGS
Molecular Pharmacology September 1965, 1 (2) 126-136;
JAMES J. BURCHALL
Wellcome Research Laboratories, Burroughs Wellcome and Co. (U.S.A.), Tuckahoe, New York
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GEORGE H. HITCHINGS
Wellcome Research Laboratories, Burroughs Wellcome and Co. (U.S.A.), Tuckahoe, New York
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Abstract

Dihydrofolate reductases have been purified approximately 100-fold from Escherichia coil, Staphylococcus aureus, and Proteus vulgaris and to a lesser degree from rat, rabbitr guinea pig, and human liver. Average Michaelis constants of 2.3 x 10-5 M for dihydrofolic acid and 1.8 x 10-5 M for NADPH have been obtained for the three bacterial enzymes. NADPH is the preferred reductant but could be replaced by NADH with about 25% efficiency. pH-activity curves, in the case of the bacterial enzymes, show a single peak with maximum activity between pH 6.8 and 7.2.

Strong correlations have been found between the binding of a drug by a particular dihydrofolate reductase and the capacity of that drug to inhibit the source organism in vitro. In addition, each dihydrofolate reductase was shown to possess a pattern of inhibition that is distinct for the species under investigation. These differences between and among bacterial and mammalian reductases may be due to changes in amino acid composition at the active sites.

ACKNOWLEDGMENTS The authors wish to thank Mr. Robert Ferone for his numerous contributions to the preparations and study of the mammalian dihydrofolate reductases and Dr. Sigmund Zakrzewski for several helpful discussions and, in particular, for his suggestion of the use of Sephadex G-75 for the purification of time bacterial dihydrofolate reductases.

  • Copyright ©, 1965, by Academic Press Inc.

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Molecular Pharmacology
Vol. 1, Issue 2
1 Sep 1965
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Research ArticleArticle

Inhibitor Binding Analysis of Dihydrofolate Reductases from Various Species

JAMES J. BURCHALL and GEORGE H. HITCHINGS
Molecular Pharmacology September 1, 1965, 1 (2) 126-136;

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Research ArticleArticle

Inhibitor Binding Analysis of Dihydrofolate Reductases from Various Species

JAMES J. BURCHALL and GEORGE H. HITCHINGS
Molecular Pharmacology September 1, 1965, 1 (2) 126-136;
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