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Molecular Pharmacology

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Research ArticleArticle

Kinetics of Cyclic Enzyme Systems

SUNGMAN CHA and CHUNG-JA MO CHA
Molecular Pharmacology September 1965, 1 (2) 178-189;
SUNGMAN CHA
Division of Medical Science, Brown University, Providence, Rhode Island
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CHUNG-JA MO CHA
Division of Medical Science, Brown University, Providence, Rhode Island
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Abstract

Kinetic rate equations which take into account the effects of depletion of free substrates and free enzymes by binding are derived for a model of cyclic enzyme systems. The reaction velocities predicted from these equations are compared with experimentally observed velocities in three different cyclic enzyme systems, i.e., pyruvic kinase and creatine kinase, pyruvic kinase and hexokinase, and pyruvic kinase and succinic thiokinase. The practical use of the last system for microdetermination of guanine nucleotides in tissue extracts is indicated.

Various applications of these equations are discussed: namely, a simple way to estimate the optimal ratio of enzymes for cycling techniques, a simple criterion for the determination of the rate-limiting enzyme in a cyclic system in vitro and in vivo, and the possible use for the determination of the molecular weight or the number of active sites per molecule of enzyme.

ACKNOWLEDGMENT The authors wish to thank Professor R. E. Parks, Jr., for his encouragement and advice, and Mrs. Mária Décsy for her excellent technical assistance. This work was supported by Grant No. T-94 G from the American Cancer Society.

  • Copyright ©, 1965, by Academic Press Inc.

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Molecular Pharmacology
Vol. 1, Issue 2
1 Sep 1965
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Research ArticleArticle

Kinetics of Cyclic Enzyme Systems

SUNGMAN CHA and CHUNG-JA MO CHA
Molecular Pharmacology September 1, 1965, 1 (2) 178-189;

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Research ArticleArticle

Kinetics of Cyclic Enzyme Systems

SUNGMAN CHA and CHUNG-JA MO CHA
Molecular Pharmacology September 1, 1965, 1 (2) 178-189;
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